Protective Effect of Neferine in Permanent Cerebral Ischemic Rats via Anti-Oxidative and Anti-Apoptotic Mechanisms.

Animals Benzylisoquinolines Brain Ischemia / metabolism Calcium / metabolism Calcium Channels Caspase 3 / metabolism Infarction, Middle Cerebral Artery / complications Neuroprotective Agents / pharmacology Nimodipine / therapeutic use Nitric Oxide / metabolism Proto-Oncogene Proteins c-bcl-2 / metabolism Rats Rats, Sprague-Dawley bcl-2-Associated X Protein / metabolism
Apoptosis Neferine Neurotoxicity Oxidative stress Permanent cerebral ischemia

Journal

Neurotoxicity research
ISSN: 1476-3524
Titre abrégé: Neurotox Res
Pays: United States
ID NLM: 100929017

Informations de publication

Date de publication:
Oct 2022
Historique:
received: 31 03 2022
accepted: 20 08 2022
revised: 10 08 2022
pubmed: 27 8 2022
medline: 30 9 2022
entrez: 26 8 2022
Statut: ppublish

Résumé

Permanent cerebral ischemia is a consequence of prolonged cerebral artery occlusion that results in severe brain damage. Neurotoxicity occurring after ischemia can induce brain tissue damage by destroying cell organelles and their function. Neferine is a natural compound isolated from the seed embryos of the lotus plant and has broad pharmacological effects, including blockading of the calcium channels, anti-oxidative stress, and anti-apoptosis. This study investigated the ability of neferine to reduce brain injury after permanent cerebral occlusion. Permanent cerebral ischemia in rats was induced by instigation of occlusion of the middle cerebral artery for 24 h. The rats were divided into 6 groups: sham, permanent middle cerebral artery occlusion (pMCAO), pMCAO with neferine and nimodipine treatment. To investigate the severity of the injury, the neurological deficit score and morphological alterations were investigated. After 24 h, the rats were evaluated to assess neurological deficit, infarct volume, morphological change, and the number of apoptotic cell deaths. In addition, the brain tissues were examined by western blot analysis to calculate the expression of proteins related to oxidative stress and apoptosis. The data showed that the neurological deficit scores and the infarct volume were significantly reduced in the neferine-treated rats compared to the vehicle group. Treatment with neferine significantly reduced oxidative stress with a measurable decrease in 4-hydroxynonenal (4-HNE), nitric oxide (NO), neuronal nitric oxide (nNOS), and calcium levels and an upregulation of Hsp70 expression. Neferine treatment also significantly decreased apoptosis, with a decrease in Bax and cleaved caspase-3 and an increase in Bcl-2. This study suggested that neferine had a neuroprotective effect on permanent cerebral ischemia in rats by diminishing oxidative stress and apoptosis.

Identifiants

DOI: 10.1007/s12640-022-00568-6 PMID: 36018507
pubmed: 36018507
doi: 10.1007/s12640-022-00568-6
pii: 10.1007/s12640-022-00568-6
doi:

Substances chimiques

Benzylisoquinolines 0
Calcium Channels 0
Neuroprotective Agents 0
Proto-Oncogene Proteins c-bcl-2 0
bcl-2-Associated X Protein 0
neferine 2292-16-2
Nitric Oxide 31C4KY9ESH
Nimodipine 57WA9QZ5WH
Caspase 3 EC 3.4.22.-
Calcium SY7Q814VUP

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1348-1359

Subventions

Organisme : Faculty of Medicine Research Fund
ID : 062-2563
Organisme : Thailand Research Fund
ID : DBG6180030

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Jirakhamon Sengking (J)

Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

Chio Oka (C)

Laboratory of Gene Function in Animals, Nara Institute of Science and Technology, 8916-5, Ikoma, Nara 630-0192, Takayama, Japan.

Nuttapong Yawoot (N)

Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

Jiraporn Tocharus (J)

Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

Waraluck Chaichompoo (W)

Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand.

Apichart Suksamrarn (A)

Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand.

Chainarong Tocharus (C)

Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. chainarongt@hotmail.com.
Center for Research and Development of Natural Products for Health, Chiang Mai University, Chiang Mai, Thailand. chainarongt@hotmail.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Isoquinoléines › Benzylisoquinoléines › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Maladies cardiovasculaires › Maladies vasculaires › Angiopathies intracrâniennes › Encéphalopathie ischémique › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Calcium › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux ioniques › Canaux calciques › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › Caspases › Caspases effectrices › Caspase-3 › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Nécrose › Infarctus › Infarctus encéphalique › Infarctus cérébral › Infarctus du territoire de l'artère cérébrale moyenne › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Agents du système nerveux central › Neuroprotecteurs › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyridines › Acides nicotiniques › Nimodipine › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Oxydes d'azote › Monoxyde d'azote › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines tumorales › Protéines oncogènes › Protéines proto-oncogènes › Protéines proto-oncogènes c-bcl-2 › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rat Sprague-Dawley › Protective Effect of Neferine in Permanent...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › Protéine Bax › Protective Effect of Neferine in Permanent...