Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 08 2022
26 08 2022
Historique:
received:
23
03
2022
accepted:
16
08
2022
entrez:
26
8
2022
pubmed:
27
8
2022
medline:
31
8
2022
Statut:
epublish
Résumé
Perforin-2 (PFN2, MPEG1) is a pore-forming protein that acts as a first line of defense in the mammalian immune system, rapidly killing engulfed microbes within the phagolysosome in macrophages. PFN2 self-assembles into hexadecameric pre-pore rings that transition upon acidification into pores damaging target cell membranes. Here, using high-speed atomic force microscopy (HS-AFM) imaging and line-scanning and molecular dynamics simulation, we elucidate PFN2 pre-pore to pore transition pathways and dynamics. Upon acidification, the pre-pore rings (pre-pore-I) display frequent, 1.8 s
Identifiants
pubmed: 36028507
doi: 10.1038/s41467-022-32757-4
pii: 10.1038/s41467-022-32757-4
pmc: PMC9418332
doi:
Substances chimiques
Pore Forming Cytotoxic Proteins
0
Perforin
126465-35-8
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5039Subventions
Organisme : NCCIH NIH HHS
ID : DP1 AT010874
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS110790
Pays : United States
Organisme : Wellcome Trust
ID : 090532/Z/09/Z
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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