Wild type and variants of SARS-COV-2 in Parisian sewage: presence in raw water and through processes in wastewater treatment plants.
COVID-19
Digital PCR
N1 gene
SARS-CoV-2
WWTPs
Wastewater surveillance
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
29
09
2021
accepted:
18
08
2022
pubmed:
28
8
2022
medline:
24
9
2022
entrez:
27
8
2022
Statut:
ppublish
Résumé
The presence of SARS-CoV-2 RNA has been extensively reported at the influent of wastewater treatment plants (WWTPs) worldwide, and its monitoring has been proposed as a potential surveillance tool to early alert of epidemic outbreaks. However, the fate of the SARS-CoV-2 RNA in the treatment process of WWTP has not been widely studied yet; therefore, in this study, we aimed to evaluate the efficiency of treatment processes in reducing SARS-CoV-2 RNA levels in wastewater. The treatment process of three WWTPs of the Parisian area in France was monitored on six different weeks over a period of 2 months (from April 14 to June 9, 2021). SARS-CoV-2 RNA copies were detected using digital polymerase chain reaction (dPCR). Investigation on the presence of variants of concern (Del69-70, E484K, and L452R) was also performed. Additionally, SARS-CoV-2 RNA loads in the WWTPs influents were expressed as the viral concentration in per population equivalent (PE) and showed a good correlation with French public health indicators (incidence rate). SARS-CoV-2 RNA loads were notably reduced along the water treatment lines of the three WWTPs studied (2.5-3.4 log reduction). Finally, very low SARS-CoV-2 RNA loads were detected in effluents (non-detected in over half of the samples) which indicated that the potential risk of the release of wastewater effluents to the environment is probably insignificant, in the case of WWTPs enabling an efficient biological removal of nitrogen.
Identifiants
pubmed: 36029443
doi: 10.1007/s11356-022-22665-x
pii: 10.1007/s11356-022-22665-x
pmc: PMC9418656
doi:
Substances chimiques
RNA, Viral
0
Sewage
0
Waste Water
0
Nitrogen
N762921K75
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
67442-67449Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Ahmed W, Angel N, Edson J, Bibby K, Bivins A, O’Brien JW, Choi PM, Kitajima M, Simpson SL, Li J, Tscharke B, Verhagen R, Smith WJM, Zaugg J, Dierens L, Hugenholtz P, Thomas KV, Mueller JF (2020a) First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: a proof of concept for the wastewater surveillance of COVID-19 in the community. Sci Total Environ 728:138764. https://doi.org/10.1016/j.scitotenv.2020.138764
doi: 10.1016/j.scitotenv.2020.138764
Ahmed W, Bertsch PM, Bivins A, Bibby K, Farkas K, Gathercole A, Haramoto E, Gyawali P, Korajkic A, McMinn BR, Mueller JF, Simpson SL, Smith WJM, Symonds EM, Thomas KV, Verhagen R, Kitajima M (2020b) Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater. Sci Total Environ 739:139960. https://doi.org/10.1016/j.scitotenv.2020.139960
doi: 10.1016/j.scitotenv.2020.139960
Ahmed W, Bivins A, Bertsch PM, Bibby K, Choi PM, Farkas K, Gyawali P, Hamilton KA, Haramoto E, Kitajima M, Simpson SL, Tandukar S, Thomas KV, Mueller JF (2020c) Surveillance of SARS-CoV-2 RNA in wastewater: methods optimization and quality control are crucial for generating reliable public health information. Curr Opin Enviro Sci Health Environ Health: COVID-19 17:82–93. https://doi.org/10.1016/j.coesh.2020c.09.003
doi: 10.1016/j.coesh.2020c.09.003
Ascoli JJ (2021) Could mutations of SARS-CoV-2 suppress diagnostic detection? Nat Biotechnol 39:270–274. https://doi.org/10.1038/s41587-021-00834-6
doi: 10.1038/s41587-021-00834-6
Balboa S, Mauricio-Iglesias M, Rodríguez S, Martínez-Lamas L, Vasallo FJ, Regueiro B, Lema JM (2020) The fate of SARS-CoV-2 in wastewater treatment plants points out the sludge line as a suitable spot for incidence monitoring (preprint). Epidemiology. https://doi.org/10.1101/2020.05.25.20112706
doi: 10.1101/2020.05.25.20112706
Bar-Or I, Weil M, Indenbaum V, Bucris E, Bar-Ilan D, Elul M, Levi N, Aguvaev I, Cohen Z, Shirazi R, Erster O, Sela-Brown A, Sofer D, Mor O, Mendelson E, Zuckerman NS (2021) Detection of SARS-CoV-2 variants by genomic analysis of wastewater samples in Israel. Sci Total Environ 789:148002. https://doi.org/10.1016/j.scitotenv.2021.148002
doi: 10.1016/j.scitotenv.2021.148002
Berestycki H, Desjardins B, Heintz B, Oury J-M (2021) The effects of heterogeneity and stochastic variability of behaviours on the intrinsic dynamics of epidemics. medRxiv 2021.03.26.21254414. https://doi.org/10.1101/2021.03.26.21254414
Bressy A, Carré C, Caupos É, de Gouvello B, Deroubaix J-F, Deutsch J-C, Mailler R, Marconi A, Neveu P, Paulic L, Pichon S, Rocher V, Severin I, Soyer M, Moilleron R (2016) Cosmet’eau—Changes in the personal care product consumption practices: from whistle-blowers to impacts on aquatic environments. Environ Sci Pollut Res 23:13581–13584. https://doi.org/10.1007/s11356-016-6794-y
doi: 10.1007/s11356-016-6794-y
Buenestado-Serrano S, Recio R, Sola Campoy PJ, Catalán P, Folgueira MD, Villa J, Muñoz Gallego I, de la Cueva VM, Meléndez MA, Andrés Zayas C, Losa-García JE, Goyanes MJ, Fraile Torres A, Von Wermitz A, Fradejas-Villajos I, del Arco C, Campelo-Gutiérrez C, González Bodi S, López-Wolf D, Iglesias-Franco H, Pérez-Lago L, Arce Arnaez A, Rodriguez Baena E, Ordobas Gavin M, Muñoz P, Delgado R, Cardeñoso L, Viedma E, García de Viedma D (2021) First confirmation of importation and transmission in Spain of the newly identified SARS-CoV-2 B.1.1.7 variant. Enfermedades Infecciosas y Microbiología Clínica S0213–005X(21):00046–X. https://doi.org/10.1016/j.eimc.2021.02.006
doi: 10.1016/j.eimc.2021.02.006
Cao Y, Raith MR, Griffith JF (2015) Droplet digital PCR for simultaneous quantification of general and human-associated fecal indicators for water quality assessment. Water Res 70:337–349. https://doi.org/10.1016/j.watres.2014.12.008
doi: 10.1016/j.watres.2014.12.008
Carcereny A, Garcia-Pedemonte D, Martínez-Velázquez A, Quer J, Garcia-Cehic D, Gregori J, Antón A, Andrés C, Pumarola T, Chacón-Villanueva C, Borrego CM, Bosch A, Guix S, Pintó RM (2022) Dynamics of SARS-CoV-2 Alpha (B.1.1.7) variant spread: the wastewater surveillance approach. Environ Res 208:112720. https://doi.org/10.1016/j.envres.2022.112720
doi: 10.1016/j.envres.2022.112720
Gasperi J, Garnaud S, Rocher V, Moilleron R (2008) Priority pollutants in wastewater and combined sewer overflow. Sci Total Environ 407:263–272. https://doi.org/10.1016/j.scitotenv.2008.08.015
doi: 10.1016/j.scitotenv.2008.08.015
Giacobbo A, Rodrigues MAS, Zoppas Ferreira J, Bernardes AM, de Pinho MN (2021) A critical review on SARS-CoV-2 infectivity in water and wastewater. What do we know? SciTotal Environ 774:145721. https://doi.org/10.1016/j.scitotenv.2021.145721
doi: 10.1016/j.scitotenv.2021.145721
Hart OE, Halden RU (2020) Computational analysis of SARS-CoV-2/COVID-19 surveillance by wastewater-based epidemiology locally and globally: feasibility, economy, opportunities and challenges. Sci Total Environ 730:138875. https://doi.org/10.1016/j.scitotenv.2020.138875
doi: 10.1016/j.scitotenv.2020.138875
Heijnen L, Elsinga G, de Graaf M, Molenkamp R, Koopmans MPG, Medema G (2021) Droplet digital RT-PCR to detect SARS-CoV-2 signature mutations of variants of concern in wastewater. Sci Total Environ 799:149456. https://doi.org/10.1016/j.scitotenv.2021.149456
doi: 10.1016/j.scitotenv.2021.149456
Hong P-Y, Rachmdi AT, Mantilla-Calderon D, Alkahtani M, Bashawri YM, Al Qarni H, M.O’Reilly K, Zhou J (2021) Estimating the minimum number of SARS-CoV-2 infected cases needed to detect viral RNA in wastewater: to what extent of the outbreak can surveillance of wastewater tell us? Environ Res 195:110748. https://doi.org/10.1016/j.envres.2021.110748
Jafferali MH, Khatami K, Atasoy M, Birgersson M, Williams C, Cetecioglu Z (2021) Benchmarking virus concentration methods for quantification of SARS-CoV-2 in raw wastewater. Sci Total Environ 755:142939. https://doi.org/10.1016/j.scitotenv.2020.142939
doi: 10.1016/j.scitotenv.2020.142939
Kitamura K, Sadamasu K, Muramatsu M, Yoshida H (2021) Efficient detection of SARS-CoV-2 RNA in the solid fraction of wastewater. Sci Total Environ 763:144587. https://doi.org/10.1016/j.scitotenv.2020.144587
doi: 10.1016/j.scitotenv.2020.144587
Kocacemi BAK, Halil K, Ahmet S, Fahriye S, Ahmet Mete S, Bekir P (2020) SARS-CoV-2 detection in Istanbul wastewater treatment plant sludges. MedRxiv 11. https://doi.org/10.1101/2020.05.12.20099358v1
Kumar M, Kuroda K, Patel AK, Patel N, Bhattacharya P, Joshi M, Joshi CG (2021a) Decay of SARS-CoV-2 RNA along the wastewater treatment outfitted with Upflow Anaerobic Sludge Blanket (UASB) system evaluated through two sample concentration techniques. Sci Total Environ 754:142329. https://doi.org/10.1016/j.scitotenv.2020.142329
doi: 10.1016/j.scitotenv.2020.142329
Kumar S, Singh R, Kumari N, Karmakar S, Behera M, Siddiqui AJ, Rajput VD, Minkina T, Bauddh K, Kumar N (2021b) Current understanding of the influence of environmental factors on SARS-CoV-2 transmission, persistence, and infectivity. Environ Sci Pollut Res 28:6267–6288. https://doi.org/10.1007/s11356-020-12165-1
doi: 10.1007/s11356-020-12165-1
Li B, Di DYW, Saingam P, Jeon MK, Yan T (2021) Fine-scale temporal dynamics of SARS-CoV-2 RNA abundance in wastewater during a COVID-19 lockdown. Water Res 197:117093. https://doi.org/10.1016/j.watres.2021.117093
doi: 10.1016/j.watres.2021.117093
Lu D, Huang Z, Luo J, Zhang X, Sha S (2020) Primary concentration – the critical step in implementing the wastewater based epidemiology for the COVID-19 pandemic: a mini-review. Sci Total Environ 747:141245. https://doi.org/10.1016/j.scitotenv.2020.141245
doi: 10.1016/j.scitotenv.2020.141245
Mailler R, Mèche P, Rocher V (2021) What removals of pathogen indicators can be expected within large-scale wastewater treatment facilities in the context of wastewater reuse in Paris conurbation? Water Sci Technol 83(4):781–791. https://doi.org/10.2166/wst.2021.004
Medema G, Heijnen L, Brouwer A, Italiaander R, Elsinga G (2020) Presence of SARS-Coronavirus‑2 RNA in sewage and correlation with reported COVID-19 prevalence in the early stage of the epidemic in the Netherlands. Environ Sci Technol Lett 7(7):511–516. https://doi.org/10.1021/acs.estlett.0c00357
Montier O, Lopez-Viveros M, Le Tallec X, Lacroix S, Tournié M, Soyeux E, Azimi S, Rocher V (2021) Comportement de l’ARN du SARS-CoV-2 au sein des filières de traitemetn des eaux et des boues du site Seine Valenton-SIAAP-SIVAL
Peterson SW, Lidder R, Daigle J, Wonitowy Q, Dueck C, Nagasawa A, Mulvey MR, Mangat CS (2022) RT-qPCR detection of SARS-CoV-2 mutations S 69–70 del, S N501Y and N D3L associated with variants of concern in Canadian wastewater samples. Sci Total Environ 810:151283. https://doi.org/10.1016/j.scitotenv.2021.151283
doi: 10.1016/j.scitotenv.2021.151283
Rački N, Morisset D, Gutierrez-Aguirre I, Ravnikar M (2014) One-step RT-droplet digital PCR: a breakthrough in the quantification of waterborne RNA viruses. Anal Bioanal Chem 406:661–667. https://doi.org/10.1007/s00216-013-7476-y
doi: 10.1007/s00216-013-7476-y
Randazzo W, Truchado P, Cuevas-Ferrando E, Simón P, Allende A, Sánchez G (2020) SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area. Water Res 181:115942. https://doi.org/10.1016/j.watres.2020.115942
doi: 10.1016/j.watres.2020.115942
Rocher V, Azimi S (2016) Qualité microbiologique des eaux en agglomération parisienne, Editions Johanet
Serra-Compte A, González S, Arnaldos M, Berlendis S, Courtois S, Loret JF, Schlosser O, Yáñez AM, Soria-Soria E, Fittipaldi M, Saucedo G, Pinar-Méndez A, Paraira M, Galofré B, Lema JM, Balboa S, Mauricio-Iglesias M, Bosch A, Pintó RM, Bertrand I, Gantzer C, Montero C, Litrico X (2021) Elimination of SARS-CoV-2 along wastewater and sludge treatment processes. Water Res 202:117435. https://doi.org/10.1016/j.watres.2021.117435
doi: 10.1016/j.watres.2021.117435
Singh M, Chazal M, Quarato P, Bourdon L, Malabat C, Vallet T, Vignuzzi M, van der Werf S, Behillil S, Donati F, Sauvonnet N, Nigro G, Bourgine M, Jouvenet N, Cecere G (2021) A virus-encoded microRNA contributes to evade innate immune response during SARS-CoV-2 infection (preprint). Mol Biol. https://doi.org/10.1101/2021.09.09.459577
doi: 10.1101/2021.09.09.459577
Staley ZR, Boyd RJ, Shum P, Edge TA (2018) Microbial source tracking using quantitative and digital PCR to identify sources of fecal contamination in stormwater, river water, and beach water in a Great Lakes Area of Concern. Appl Environ Microbiol 84. https://doi.org/10.1128/AEM.01634-18
WHO (2020) Status of environmental surveillance for SARS-CoV-2 virus
Wurtzer S, Marechal V, Mouchel J-M, Maday Y, Teyssou R, Richard E, Almayrac JL, Moulin L (2020) Evaluation of lockdown impact on SARS-CoV-2 dynamics through viral genome quantification in Paris wastewaters (preprint). Epidemiology. https://doi.org/10.1101/2020.04.12.20062679
doi: 10.1101/2020.04.12.20062679
Wurtzer S, Waldman P, Levert M, Cluzel N, Almayrac JL, Charpentier C, Masnada S, Gillon-Ritz M, Mouchel JM, Maday Y, Boni M, Marechal V, Moulin L (2022) SARS-CoV-2 genome quantification in wastewaters at regional and city scale allows precise monitoring of the whole outbreaks dynamics and variants spreading in the population. Sci Total Environ 810:152213. https://doi.org/10.1016/j.scitotenv.2021.152213
doi: 10.1016/j.scitotenv.2021.152213
Yaniv K, Ozer E, Shagan M, Lakkakula S, Plotkin N, Bhandarkar NS, Kushmaro A (2021) Direct RT-qPCR assay for SARS-CoV-2 variants of concern (Alpha, B.1.1.7 and Beta, B.1.351) detection and quantification in wastewater. Environ Res 201:111653. https://doi.org/10.1016/j.envres.2021a.111653
doi: 10.1016/j.envres.2021a.111653
Yaniv K, Shagan M, Lewis YE, Kramarsky-Winter E, Weil M, Indenbaum V, Elul M, Erster O, Brown AS, Mendelson E, Mannasse B, Shirazi R, Lakkakula S, Miron O, Rinott E, Baibich RG, Bigler I, Malul M, Rishti R, Brenner A, Friedler E, Gilboa Y, Sabach S, Alfiya Y, Cheruti U, Nadav D, Moran-Gilad J, Berchenko Y, Bar-Or I, Kushmaro A (2021) City-level SARS-CoV-2 sewage surveillance. Chemosphere 283:131194. https://doi.org/10.1016/j.chemosphere.2021.131194
doi: 10.1016/j.chemosphere.2021.131194