Determination of an RT-qPCR viral load cutoff point for the etiologic diagnosis of rotavirus A diarrhea in neonate dairy calves.
RT-qPCR—real-time quantitative polymerase chain reaction
clinical outcome
cutoff point
dairy calves
enzyme-linked immunosorbent assay (ELISA)
neonatal calf diarrhea
rotavirus A
viral load
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2022
2022
Historique:
received:
24
05
2022
accepted:
27
07
2022
entrez:
29
8
2022
pubmed:
30
8
2022
medline:
30
8
2022
Statut:
epublish
Résumé
Rotavirus A (RVA) is amongst the most widespread causes of neonatal calf diarrhea. Because subclinical infections are common, the diagnosis of RVA-induced diarrhea cannot rely solely on molecular viral detection. However, RT-qPCR allows for quantification of RVA shedding in feces, which can be correlated with clinical disease. Here, we determine an optimal cutoff of rotaviral load quantified by RT-qPCR to predict RVA causality in diarrheic neonate calves, using RVA antigen-capture ELISA as reference test. Feces from 328 diarrheic (
Identifiants
pubmed: 36032290
doi: 10.3389/fvets.2022.952197
pmc: PMC9411863
doi:
Types de publication
Journal Article
Langues
eng
Pagination
952197Informations de copyright
Copyright © 2022 Caffarena, Castells, Schild, Casaux, Armendano, Colina and Giannitti.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The handling editor EU declared a past co-authorship with the author FG.
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