Cloning of a novel tetrahydrofolate-dependent dicamba demethylase gene from dicamba-degrading consortium and characterization of the gene product.

Dmt06 dicamba gene clone microbial consortium tetrahydrofolate-dependent demethylase

Journal

Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977

Informations de publication

Date de publication:
2022
Historique:
received: 26 06 2022
accepted: 14 07 2022
entrez: 29 8 2022
pubmed: 30 8 2022
medline: 30 8 2022
Statut: epublish

Résumé

Dicamba, an important hormone-type systemic herbicide, is widely used to control more than 200 kinds of broadleaf weeds in agriculture. Due to its broad-spectrum, high efficiency and effectively killing glyphosate-resistant weeds, dicamba is considered as an excellent target herbicide for the engineering of herbicide-resistant crops. In this study, an efficient dicamba-degrading microbial consortium was enriched from soil collected from the outfall of a pesticide factory. The enriched consortium could almost completely degrade 500 mg/L of dicamba within 12 h of incubation. A novel tetrahydrofolate (THF)-dependent dicamba demethylase gene, named

Identifiants

pubmed: 36033860
doi: 10.3389/fmicb.2022.978577
pmc: PMC9404685
doi:

Types de publication

Journal Article

Langues

eng

Pagination

978577

Informations de copyright

Copyright © 2022 Li, Chen, Chen, Yuan, Zhang and He.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Na Li (N)

College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, China.
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.

Le Chen (L)

Jiangsu Academy of Agricultural Sciences, Institute of Germplasm Resources and Biotechnology, Nanjing, China.

E Chen (E)

The Environmental Monitoring Center of Gansu Province, Lanzhou, China.

Cansheng Yuan (C)

College of Rural Revitalization, Jiangsu Open University, Nanjing, China.

Hao Zhang (H)

College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, China.

Jian He (J)

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
College of Rural Revitalization, Jiangsu Open University, Nanjing, China.

Classifications MeSH