Microbial Aerosols Generated from Standard Microbiological Laboratory Procedures.
aerosol generation
bacterial spore
laboratory procedures
pipetting
spillage
Journal
Applied biosafety : journal of the American Biological Safety Association
ISSN: 2470-1246
Titre abrégé: Appl Biosaf
Pays: United States
ID NLM: 101122979
Informations de publication
Date de publication:
01 Jun 2022
01 Jun 2022
Historique:
entrez:
29
8
2022
pubmed:
30
8
2022
medline:
30
8
2022
Statut:
ppublish
Résumé
Modern microbiology laboratories are designed to protect workers and the environment from microbial aerosols produced during microbiological procedures and accidents. However, there is only limited data available on the aerosols generated from common microbiology procedures. A series of common microbiological procedures were undertaken with high concentration spore suspensions while air samplers were operated to sample the aerosols generated. Surface contamination from droplets was visualized using sodium fluorescein within the suspension. A total of 36 procedures were studied using different sample volumes (0.1-10 mL) and two spore suspension titers (10 The aerosol concentrations generated varied from 0 to 13,000 cfu/m Aerosol generation from common laboratory processes can be minimized by reducing sample volumes and concentrations if possible. Training laboratory staff in good microbiological techniques would further mitigate aerosols generated from common laboratory processes.
Sections du résumé
Background
UNASSIGNED
Modern microbiology laboratories are designed to protect workers and the environment from microbial aerosols produced during microbiological procedures and accidents. However, there is only limited data available on the aerosols generated from common microbiology procedures.
Methods
UNASSIGNED
A series of common microbiological procedures were undertaken with high concentration spore suspensions while air samplers were operated to sample the aerosols generated. Surface contamination from droplets was visualized using sodium fluorescein within the suspension. A total of 36 procedures were studied using different sample volumes (0.1-10 mL) and two spore suspension titers (10
Results
UNASSIGNED
The aerosol concentrations generated varied from 0 to 13,000 cfu/m
Conclusions
UNASSIGNED
Aerosol generation from common laboratory processes can be minimized by reducing sample volumes and concentrations if possible. Training laboratory staff in good microbiological techniques would further mitigate aerosols generated from common laboratory processes.
Identifiants
pubmed: 36035500
doi: 10.1089/apb.2021.0038
pii: 10.1089/apb.2021.0038
pmc: PMC9150131
doi:
Types de publication
Journal Article
Langues
eng
Pagination
92-99Subventions
Organisme : World Health Organization
ID : 001
Pays : International
Informations de copyright
© Thomas Pottage et al. 2022; Published by Mary Ann Liebert, Inc.
Déclaration de conflit d'intérêts
No competing financial interests exist.
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