Unraveling the Photoactivation Mechanism of a Light-Activated Adenylyl Cyclase Using Ultrafast Spectroscopy Coupled with Unnatural Amino Acid Mutagenesis.

Adenosine Triphosphate Adenylyl Cyclases / genetics Amino Acids Bacterial Proteins / metabolism Flavin-Adenine Dinucleotide / chemistry Flavins / chemistry Light Mutagenesis Protons Spectrum Analysis Tyrosine

Journal

ACS chemical biology
ISSN: 1554-8937
Titre abrégé: ACS Chem Biol
Pays: United States
ID NLM: 101282906

Informations de publication

Date de publication:
16 09 2022
Historique:
pubmed: 30 8 2022
medline: 20 9 2022
entrez: 29 8 2022
Statut: ppublish

Résumé

The hydrogen bonding network that surrounds the flavin in blue light using flavin adenine dinucleotide (BLUF) photoreceptors plays a crucial role in sensing and communicating the changes in the electronic structure of the flavin to the protein matrix upon light absorption. Using time-resolved infrared spectroscopy (TRIR) and unnatural amino acid incorporation, we investigated the photoactivation mechanism and the role of the conserved tyrosine (Y6) in the forward reaction of the photoactivated adenylyl cyclase from

Identifiants

DOI: 10.1021/acschembio.2c00575 PMID: 36038143 PMC: PMC9486806
pubmed: 36038143
doi: 10.1021/acschembio.2c00575
pmc: PMC9486806
doi:

Substances chimiques

Amino Acids 0
Bacterial Proteins 0
Flavins 0
Protons 0
Flavin-Adenine Dinucleotide 146-14-5
Tyrosine 42HK56048U
Adenosine Triphosphate 8L70Q75FXE
Adenylyl Cyclases EC 4.6.1.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

2643-2654

Subventions

Organisme : NIGMS NIH HHS
ID : K12 GM102778
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM092714
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM135746
Pays : United States

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Auteurs

Jinnette Tolentino Collado (J)

Department of Chemistry, Stony Brook University, New York, New York 11794, United States.

James N Iuliano (JN)

Department of Chemistry, Stony Brook University, New York, New York 11794, United States.
ORCID: 0000-0003-1213-3292

Katalin Pirisi (K)

Department of Biophysics, Medical School, University of Pecs, Szigeti Street 12, Pecs 7624, Hungary.

Samruddhi Jewlikar (S)

Department of Chemistry, Stony Brook University, New York, New York 11794, United States.

Katrin Adamczyk (K)

School of Chemistry, University of East Anglia, Norwich NR4 7TJ, U.K.

Gregory M Greetham (GM)

Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot OX11 0QX, U.K.

Michael Towrie (M)

Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot OX11 0QX, U.K.

Jeremy R H Tame (JRH)

Drug Design Laboratory, Graduate School of Medical Life Science, Yokohama City University, Tsurumi, Yokohama 230-0045, Japan.
ORCID: 0000-0002-9341-7280

Stephen R Meech (SR)

School of Chemistry, University of East Anglia, Norwich NR4 7TJ, U.K.
ORCID: 0000-0001-5561-2782

Peter J Tonge (PJ)

Department of Chemistry, Stony Brook University, New York, New York 11794, United States.
ORCID: 0000-0003-1606-3471

Andras Lukacs (A)

Department of Biophysics, Medical School, University of Pecs, Szigeti Street 12, Pecs 7624, Hungary.
ORCID: 0000-0001-8841-9823

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › Adénosine triphosphate › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Adenylate Cyclase › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Flavines › Riboflavine › Flavine adénine dinucléotide › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Flavines › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Lumière › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Phénomènes physiques › Particules élémentaires › Nucléons › Protons › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Unraveling the Photoactivation Mechanism of a...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés cycliques › Acides aminés aromatiques › Tyrosine › Unraveling the Photoactivation Mechanism of a...