Label-free electrochemical immunosensor for picomolar detection of the cervical cancer biomarker MCM5.
Cancer biomarkers
Charge-transfer resistance
Cyclic voltammetry
Gold disk electrode
Impedance spectroscopy
MiniChromosome maintenance protein 5
Self-assembled monolayers
Journal
Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534
Informations de publication
Date de publication:
08 Sep 2022
08 Sep 2022
Historique:
received:
31
05
2022
revised:
28
07
2022
accepted:
31
07
2022
entrez:
29
8
2022
pubmed:
30
8
2022
medline:
1
9
2022
Statut:
ppublish
Résumé
An immunosensor for label-free electrochemical detection of MiniChromosome Maintenance Protein 5, MCM5, a protein overexpressed in cervical cancer, based on a gold electrode is reported. The electrode was first modified with a submonolayer (capture layer) of 11-mercaptoundecanoic acid (11-MUA) and then activated with N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to immobilize the capture antibody. The change in electrode surface properties (wettability) during the formation of the 11-MUA layers was determined using the static water contact angle (WCA). The binding of MCM5 antigens to the capture antibody was monitored by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) using 5 mM [Fe(CN)
Identifiants
pubmed: 36038236
pii: S0003-2670(22)00797-8
doi: 10.1016/j.aca.2022.340226
pii:
doi:
Substances chimiques
Biomarkers, Tumor
0
Cell Cycle Proteins
0
MCM5 protein, human
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
340226Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.