Single-Particle Tracking and Trajectory Analysis of Fluorescent Nanodiamonds in Cell-Free Environment and Live Cells.
fluorescent nanodiamonds
free radicals
magnetometry
single-particle tracking
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
revised:
26
07
2022
received:
03
03
2022
pubmed:
30
8
2022
medline:
30
9
2022
entrez:
29
8
2022
Statut:
ppublish
Résumé
Diamond magnetometry can provide new insights on the production of free radicals inside live cells due to its high sensitivity and spatial resolution. However, the measurements often lack intracellular context for the recorded signal. In this paper, the possible use of single-particle tracking and trajectory analysis of fluorescent nanodiamonds (FNDs) to bridge that gap is explored. It starts with simulating a set of different possible scenarios of a particle's movement, reflecting different modes of motion, degrees of confinement, as well as shapes and sizes of that confinement. Then, the insights from the analysis of the simulated trajectories are applied to describe the movement of FNDs in glycerol solutions. It is shown that the measurements are in good agreement with the previously reported findings and that trajectory analysis yields meaningful results, when FNDs are tracked in a simple environment. Then the much more complex situation of FNDs moving inside a live cell is focused. The behavior of the particles after different incubation times is analyzed, and the possible intracellular localization of FNDs is deducted from their trajectories. Finally, this approach is combined with long-term magnetometry methods to obtain maps of the spin relaxation dynamics (or T1) in live cells, as FNDs move through the cytosol.
Identifiants
pubmed: 36038355
doi: 10.1002/smll.202201395
doi:
Substances chimiques
Fluorescent Dyes
0
Nanodiamonds
0
Diamond
7782-40-3
Glycerol
PDC6A3C0OX
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2201395Subventions
Organisme : European Research Council
ID : ERC-2016-STG
Pays : International
Organisme : European Research Council
ID : 714289
Pays : International
Informations de copyright
© 2022 The Authors. Small published by Wiley-VCH GmbH.
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