Osteogenic transdifferentiation of primary human fibroblasts to osteoblast-like cells with human platelet lysate.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 08 2022
29 08 2022
Historique:
received:
12
09
2017
accepted:
10
08
2022
entrez:
29
8
2022
pubmed:
30
8
2022
medline:
1
9
2022
Statut:
epublish
Résumé
Inherited bone disorders account for about 10% of documented Mendelian disorders and are associated with high financial burden. Their study requires osteoblasts which play a critical role in regulating the development and maintenance of bone tissue. However, bone tissue is not always available from patients. We developed a highly efficient platelet lysate-based approach to directly transdifferentiate skin-derived human fibroblasts to osteoblast-like cells. We extensively characterized our in vitro model by examining the expression of osteoblast-specific markers during the transdifferentiation process both at the mRNA and protein level. The transdifferentiated osteoblast-like cells showed significantly increased expression of a panel of osteogenic markers. Mineral deposition and ALP activity were also shown, confirming their osteogenic properties. RNA-seq analysis allowed the global study of changes in the transcriptome of the transdifferentiated cells. The transdifferentiated cells clustered separately from the primary fibroblasts with regard to the significantly upregulated genes indicating a distinct transcriptome profile; transdifferentiated osteoblasts also showed significant enrichment in gene expression related to skeletal development and bone mineralization. Our presented in vitro model may potentially contribute to the prospect of studying osteoblast-dependent disorders in patient-derived cells.
Identifiants
pubmed: 36038602
doi: 10.1038/s41598-022-18512-1
pii: 10.1038/s41598-022-18512-1
pmc: PMC9424276
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14686Informations de copyright
© 2022. The Author(s).
Références
J Nippon Med Sch. 2010 Feb;77(1):4-12
pubmed: 20154452
Connect Tissue Res. 2014 Aug;55 Suppl 1:83-7
pubmed: 25158187
J Transl Med. 2011 Mar 22;9:29
pubmed: 21418664
J Cell Biol. 2004 Jul 5;166(1):85-95
pubmed: 15226309
Biochem Biophys Res Commun. 2015 Nov 27;467(4):1110-6
pubmed: 26499074
Biosci Rep. 2015 Apr 28;35(2):
pubmed: 25797907
Open Biol. 2015 Feb;5(2):140201
pubmed: 25652541
J Bone Miner Res. 2009 Nov;24(11):1927-35
pubmed: 19419324
World J Stem Cells. 2016 Feb 26;8(2):47-55
pubmed: 26981170
Stem Cell Res Ther. 2013;4(5):117
pubmed: 24073831
Oral Maxillofac Surg Clin North Am. 2010 Aug;22(3):283-93, v
pubmed: 20713262
J Histochem Cytochem. 2007 Nov;55(11):1095-104
pubmed: 17625229
Cell. 2006 Aug 25;126(4):663-76
pubmed: 16904174
Am J Med Genet A. 2015 Dec;167A(12):2869-92
pubmed: 26394607
J Orthop Surg Res. 2010 Jun 15;5:37
pubmed: 20550694
Biotechnol Prog. 2006 Nov-Dec;22(6):1697-701
pubmed: 17137320
Blood Rev. 2013 Mar;27(2):77-89
pubmed: 23434399
J Cell Physiol. 2018 Dec;233(12):9099-9109
pubmed: 29943820
BMC Genomics. 2011 Jun 06;12:293
pubmed: 21645359
J Bone Miner Res. 1994 Nov;9(11):1697-704
pubmed: 7863820
Sci Rep. 2019 Feb 11;9(1):1761
pubmed: 30741963
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Plant J. 2015 Nov;84(3):491-503
pubmed: 26331235
Front Biosci (Landmark Ed). 2011 Jun 01;16(7):2598-621
pubmed: 21622198
Nat Rev Mol Cell Biol. 2011 Dec 22;13(1):27-38
pubmed: 22189423
Cell Physiol Biochem. 2014;34(3):703-14
pubmed: 25171291
Genome Biol. 2014 Mar 21;15(3):R52
pubmed: 24655370
Osteoporos Int. 2014 Mar;25(3):887-95
pubmed: 24114401
Osteoporos Int. 2003;14 Suppl 3:S35-42
pubmed: 12730768
J Biol Chem. 2012 Jan 6;287(2):905-15
pubmed: 22102412
Adv Exp Med Biol. 2010;658:43-9
pubmed: 19950014
Front Endocrinol (Lausanne). 2020 Jun 19;11:394
pubmed: 32636804
Proc Nutr Soc. 2007 May;66(2):158-65
pubmed: 17466098
Sci Rep. 2016 May 26;6:26761
pubmed: 27225733
J Cell Physiol. 2006 Dec;209(3):935-42
pubmed: 16972259
Adv Skin Wound Care. 2011 Aug;24(8):357-68
pubmed: 21768787
Haematologica. 2009 Feb;94(2):258-63
pubmed: 19109217
J Cell Biochem. 2016 Nov;117(11):2538-45
pubmed: 26990860
J Craniomaxillofac Surg. 2013 Mar;41(2):153-61
pubmed: 22878221
Curr Osteoporos Rep. 2012 Jun;10(2):118-25
pubmed: 22552701
Sci Rep. 2015 Dec 04;5:17518
pubmed: 26634432
Regen Ther. 2020 Nov 01;15:226-235
pubmed: 33426223
Nucleic Acids Res. 2010 Jul;38(12):e131
pubmed: 20395217
Stem Cell Res Ther. 2012 Feb 14;3(1):6
pubmed: 22333342
Nat Rev Genet. 2011 Feb;12(2):87-98
pubmed: 21191423
Osteoporos Int. 2015 Oct;26(10):2529-58
pubmed: 26070300
Bone. 2016 Mar;84:169-180
pubmed: 26769004
Bone Res. 2013 Jun 28;1(2):107-19
pubmed: 26273496
Anal Biochem. 2004 Jun 1;329(1):77-84
pubmed: 15136169
Biol Cell. 2011 Apr;103(4):197-208
pubmed: 21332447
Stem Cells Transl Med. 2014 Jul;3(7):867-78
pubmed: 24855277
Hum Mutat. 2017 Apr;38(4):439-450
pubmed: 28074631
Stem Cells Int. 2016;2016:9458396
pubmed: 27994625
Front Physiol. 2014 Apr 09;5:137
pubmed: 24782782
Cell. 1997 May 30;89(5):755-64
pubmed: 9182763
Biomaterials. 2010 Jan;31(2):270-8
pubmed: 19783038
Stem Cells Transl Med. 2020 Mar;9(3):403-415
pubmed: 31904196
J Nucl Med. 2009 May;50(5):765-73
pubmed: 19372470