Electron microscopy of cellular ultrastructure in three dimensions.

Cryoelectron Microscopy / methods Electron Microscope Tomography / methods Imaging, Three-Dimensional / methods Microscopy, Electron

Journal

Current opinion in structural biology
ISSN: 1879-033X
Titre abrégé: Curr Opin Struct Biol
Pays: England
ID NLM: 9107784

Informations de publication

Date de publication:
10 2022
Historique:
received: 31 03 2022
revised: 05 07 2022
accepted: 19 07 2022
pubmed: 31 8 2022
medline: 28 9 2022
entrez: 30 8 2022
Statut: ppublish

Résumé

Electron microscopy in three dimensions (3D) of cells and tissues can be essential for understanding the ultrastructural aspects of biological processes. The quest for 3D information reveals challenges at many stages of the workflow, from sample preparation, to imaging, data analysis and segmentation. Here, we outline several available methods, including volume SEM imaging, cryo-TEM and cryo-STEM tomography, each one occupying a different domain in the basic tradeoff between field-of-view and resolution. We discuss the considerations for choosing a suitable method depending on research needs and highlight recent developments that are essential for making 3D volume imaging of cells and tissues a standard tool for cellular and structural biologists.

Identifiants

DOI: 10.1016/j.sbi.2022.102444 PMID: 36041268
pubmed: 36041268
pii: S0959-440X(22)00123-3
doi: 10.1016/j.sbi.2022.102444
pii:
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

102444

Informations de copyright

Copyright © 2022 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest statement Nothing declared.

Auteurs

Neta Varsano (N)

Department of Chemical Research Support, Weizmann Institute of Science, 234 Herzl St., Rehovot 76100, Israel.

Sharon Grayer Wolf (SG)

Department of Chemical Research Support, Weizmann Institute of Science, 234 Herzl St., Rehovot 76100, Israel. Electronic address: sharon.wolf@weizmann.ac.il.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Electron microscopy of cellular ultrastructure...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à transmission › Tomographie en microscopie électronique › Electron microscopy of cellular ultrastructure...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Electron microscopy of cellular ultrastructure...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Electron microscopy of cellular ultrastructure...