Phenotypic drug screen uncovers the metabolic GCH1/BH4 pathway as key regulator of EGFR/KRAS-mediated neuropathic pain and lung cancer.


Journal

Science translational medicine
ISSN: 1946-6242
Titre abrégé: Sci Transl Med
Pays: United States
ID NLM: 101505086

Informations de publication

Date de publication:
31 08 2022
Historique:
entrez: 31 8 2022
pubmed: 1 9 2022
medline: 9 9 2022
Statut: ppublish

Résumé

Increased tetrahydrobiopterin (BH4) generated in injured sensory neurons contributes to increased pain sensitivity and its persistence. GTP cyclohydrolase 1 (GCH1) is the rate-limiting enzyme in the de novo BH4 synthetic pathway, and human single-nucleotide polymorphism studies, together with mouse genetic modeling, have demonstrated that decreased GCH1 leads to both reduced BH4 and pain. However, little is known about the regulation of

Identifiants

pubmed: 36044597
doi: 10.1126/scitranslmed.abj1531
pmc: PMC9985140
mid: NIHMS1878035
doi:

Substances chimiques

Analgesics 0
KRAS protein, human 0
Biopterins 0
EGFR protein, human EC 2.7.10.1
ErbB Receptors EC 2.7.10.1
GTP Cyclohydrolase EC 3.5.4.16
Gch1 protein, mouse EC 3.5.4.16
Proto-Oncogene Proteins p21(ras) EC 3.6.5.2
sapropterin EGX657432I

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

eabj1531

Subventions

Organisme : British Heart Foundation
ID : CH/16/1/32013
Pays : United Kingdom
Organisme : NINDS NIH HHS
ID : R01 NS112266
Pays : United States
Organisme : NINDS NIH HHS
ID : R35 NS105076
Pays : United States
Organisme : Austrian Science Fund FWF
ID : Z 271
Pays : Austria

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Auteurs

Shane J F Cronin (SJF)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.
Institute of Molecular Biotechnology Austria (IMBA), Dr. Bohrgasse 3, Vienna A-1030, Austria.

Shuan Rao (S)

Institute of Molecular Biotechnology Austria (IMBA), Dr. Bohrgasse 3, Vienna A-1030, Austria.

Miguel A Tejada (MA)

Institute of Molecular Biotechnology Austria (IMBA), Dr. Bohrgasse 3, Vienna A-1030, Austria.

Bruna Lenfers Turnes (BL)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.

Simon Licht-Mayer (S)

Institute of Molecular Biotechnology Austria (IMBA), Dr. Bohrgasse 3, Vienna A-1030, Austria.

Takao Omura (T)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.

Christian Brenneis (C)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.

Emily Jacobs (E)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.

Lee Barrett (L)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.

Alban Latremoliere (A)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.
Departments of Neurosurgery and Neuroscience, Neurosurgery Pain Research Institute, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.

Nick Andrews (N)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.

Keith M Channon (KM)

Radcliffe Department of Medicine, British Heart Foundation Centre of Research Excellence, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK.

Alexandra Latini (A)

LABOX, Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brazil.

Anthony C Arvanites (AC)

Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Ave, Cambridge, MA 02138, USA.
Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.

Lance S Davidow (LS)

Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Ave, Cambridge, MA 02138, USA.
Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.

Michael Costigan (M)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.

Lee L Rubin (LL)

Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Ave, Cambridge, MA 02138, USA.
Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.

Josef M Penninger (JM)

Institute of Molecular Biotechnology Austria (IMBA), Dr. Bohrgasse 3, Vienna A-1030, Austria.
Department of Medical Genetics, Life Sciences Institute, UBC, Vancouver, BC V6T 1Z3, Canada.

Clifford J Woolf (CJ)

Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA.

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