nNOS-derived NO modulates force production and iNO-derived NO the excitability in C2C12-derived 3D tissue engineering skeletal muscle
C2C12
calcium
force
myooid
nitric oxide
tissue engineering
Journal
Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006
Informations de publication
Date de publication:
2022
2022
Historique:
received:
17
05
2022
accepted:
14
07
2022
entrez:
1
9
2022
pubmed:
2
9
2022
medline:
2
9
2022
Statut:
epublish
Résumé
Nitric oxide (NO) is a bioactive gas produced by one of the three NO synthases: neuronal NOS (nNOS), inducible (iNOS), and endothelial NOS (eNOS). NO has a relevant modulatory role in muscle contraction; this takes place through two major signaling pathways: (i) activation of soluble guanylate cyclase and, thus, protein kinase G or (ii) nitrosylation of sulfur groups of cysteine. Although it has been suggested that nNOS-derived NO is the responsible isoform in muscle contraction, the roles of eNOS and iNOS and their signaling pathways have not yet been clarified. To elucidate the action of each pathway, we optimized the generation of myooids, an engineered skeletal muscle tissue based on the C2C12 cell line. In comparison with diaphragm strips from wild-type mice, 180 myooids were analyzed, which expressed all relevant excitation-contraction coupling proteins and both nNOS and iNOS isoforms. Along with the biochemical results, myooids treated with NO donor (SNAP) and unspecific NOS blocker (L-NAME) revealed a comparable NO modulatory effect on force production as was observed in the diaphragm strips. Under the effects of pharmacological tools, we analyzed the myooids in response to electrical stimulation of two possible signaling pathways and NO sources. The nNOS-derived NO exerted its negative effect on force production
Identifiants
pubmed: 36045747
doi: 10.3389/fphys.2022.946682
pii: 946682
pmc: PMC9421439
doi:
Types de publication
Journal Article
Langues
eng
Pagination
946682Informations de copyright
Copyright © 2022 Mosqueira, Scheid, Kiemel, Richardt, Rheinberger, Ollech, Lutge, Heißenberg, Pfitzer, Engelskircher, Yildiz and Porth.
Déclaration de conflit d'intérêts
Author L-MS was employed by the company PromoCell GmbH. Author LP was employed by the company myNEO NV. Author LE was employed by the company Immatics Biotechnology GmbH. The remaining author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
SLAS Discov. 2019 Jan;24(1):47-56
pubmed: 30102873
Acta Neuropathol. 1997 Nov;94(5):455-64
pubmed: 9386778
J Vis Exp. 2013 Mar 19;(73):e4267
pubmed: 23542531
In Vitro Cell Dev Biol Anim. 2000 May;36(5):327-35
pubmed: 10937836
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11158-62
pubmed: 11562475
J Tissue Eng Regen Med. 2008 Jul;2(5):279-87
pubmed: 18512267
Redox Biol. 2013 Oct 28;1:514-26
pubmed: 24251120
Circ Res. 2003 Jun 27;92(12):1322-9
pubmed: 12764022
Front Physiol. 2017 Aug 23;8:607
pubmed: 28878687
PLoS One. 2020 Dec 9;15(12):e0243660
pubmed: 33296434
Sci Rep. 2020 Jan 14;10(1):288
pubmed: 31937888
Med Res Rev. 2013 Sep;33(5):1174-213
pubmed: 23633235
J Pediatr Surg. 2003 Sep;38(9):1348-53
pubmed: 14523818
Acta Physiol Scand. 1998 Mar;162(3):401-9
pubmed: 9578386
J Vis Exp. 2013 Jan 31;(71):e50036
pubmed: 23407283
Biotechnol Bioeng. 2009 Aug 1;103(5):1034-41
pubmed: 19350625
J Biomech. 2010 May 28;43(8):1514-21
pubmed: 20189177
J Cell Physiol. 2015 Oct;230(10):2489-97
pubmed: 25857846
Traffic. 2000 Apr;1(4):326-41
pubmed: 11208118
Microsurgery. 2005;25(4):338-45
pubmed: 15880470
Am J Physiol. 1999 Sep;277(3):H1167-77
pubmed: 10484439
J Biosci Bioeng. 2011 Sep;112(3):273-8
pubmed: 21646045
J Biol Chem. 2006 Dec 29;281(52):40354-68
pubmed: 17071618
Antioxid Redox Signal. 2000 Spring;2(1):35-40
pubmed: 11232597
Cell Tissue Res. 1998 Jan;291(1):57-63
pubmed: 9394043
Biochem J. 1997 May 15;324 ( Pt 1):237-42
pubmed: 9164862
Am J Physiol Regul Integr Comp Physiol. 2000 Jan;278(1):R95-R100
pubmed: 10644626
Am J Physiol. 1991 Mar;260(3 Pt 1):C475-84
pubmed: 2003574
Am J Physiol Cell Physiol. 2017 Mar 1;312(3):C316-C327
pubmed: 27974300
FASEB J. 1991 Oct;5(13):2860-7
pubmed: 1916108
Biochem J. 2013 Jan 1;449(1):133-42
pubmed: 23009292
Tissue Eng Part A. 2015 Oct;21(19-20):2595-604
pubmed: 26166548
Biochem J. 1999 Jun 15;340 ( Pt 3):657-69
pubmed: 10359649
Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9142-7
pubmed: 8799168
J Appl Physiol (1985). 1998 Dec;85(6):2330-6
pubmed: 9843560
J Physiol. 2011 May 1;589(Pt 9):2181-96
pubmed: 21115647
Tissue Eng Part C Methods. 2009 Sep;15(3):501-11
pubmed: 19191517
BMC Musculoskelet Disord. 2020 Jul 21;21(1):479
pubmed: 32693782
Nat Med. 2009 Mar;15(3):325-30
pubmed: 19198614
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9265-9
pubmed: 2827174
Cell. 2000 Aug 18;102(4):499-509
pubmed: 10966111
J Biosci Bioeng. 2013 Feb;115(2):115-21
pubmed: 23026451
J Biol Chem. 1994 May 13;269(19):13725-8
pubmed: 7514592
J Pathol. 2011 Jan;223(1):88-98
pubmed: 21125668
Sci Rep. 2016 Jan 25;6:19614
pubmed: 26804343
J Appl Physiol (1985). 1996 Dec;81(6):2415-20
pubmed: 9018487
J Cell Biochem. 2014 Dec;115(12):2198-207
pubmed: 25146978
J Biomech. 1991;24 Suppl 1:91-9
pubmed: 1791184
Front Biosci. 2000 May 01;5:D504-26
pubmed: 10799358
J Interferon Cytokine Res. 2010 May;30(5):321-7
pubmed: 20035621
Microcirculation. 2011 Aug;18(6):501-11
pubmed: 21535296
J Exp Med. 2008 Sep 1;205(9):2053-63
pubmed: 18695006
PLoS One. 2015 Oct 29;10(10):e0141572
pubmed: 26513461
J Biol Chem. 1999 Dec 24;274(52):36831-4
pubmed: 10601232
FASEB J. 1996 Dec;10(14):1614-20
pubmed: 9002553
Chem Rev. 2018 Feb 14;118(3):1338-1408
pubmed: 29400454
J Physiol. 2008 Sep 1;586(17):4209-23
pubmed: 18617565
J Membr Biol. 2000 Feb 1;173(3):227-36
pubmed: 10667918
ACS Appl Mater Interfaces. 2017 Dec 13;9(49):42444-42458
pubmed: 29023089
J Mol Cell Cardiol. 2013 Jul;60:107-15
pubmed: 23624088
J Tissue Eng Regen Med. 2011 Jul;5(7):529-39
pubmed: 21695794
J Tissue Eng. 2021 Feb 10;12:2041731420981339
pubmed: 33628411
Tissue Eng Part A. 2022 Mar;28(5-6):270-282
pubmed: 34375125
Biochem J. 1994 Mar 1;298 ( Pt 2):249-58
pubmed: 7510950
Cell Tissue Res. 1998 May;292(2):293-302
pubmed: 9560472
Biochem Biophys Res Commun. 2005 May 6;330(2):615-21
pubmed: 15796928
Am J Physiol Endocrinol Metab. 2016 May 15;310(10):E838-45
pubmed: 27006199
Am J Physiol Heart Circ Physiol. 2002 Feb;282(2):H429-36
pubmed: 11788389
Sci Rep. 2019 Dec 19;9(1):19453
pubmed: 31857625
J Physiol. 1998 Jun 1;509 ( Pt 2):577-86
pubmed: 9575305
Adv Exp Med Biol. 2003;536:345-51
pubmed: 14635687
J Biol Chem. 2003 Mar 7;278(10):8184-9
pubmed: 12509428
Biotechnol Bioeng. 2010 Apr 15;105(6):1161-7
pubmed: 20014142
Pharmacol Rev. 2010 Sep;62(3):525-63
pubmed: 20716671
Basic Res Cardiol. 2021 Mar 17;116(1):18
pubmed: 33728868
J Physiol. 2009 Feb 1;587(3):655-68
pubmed: 19047200
J Cell Physiol. 2017 Oct;232(10):2788-2797
pubmed: 28409828
Am J Physiol Cell Physiol. 2001 Feb;280(2):C288-95
pubmed: 11208523
J Tissue Eng Regen Med. 2010 Aug;4(6):437-43
pubmed: 20084621
Hum Gene Ther. 1996 Nov 10;7(17):2195-200
pubmed: 8934233
Plast Reconstr Surg. 2004 Feb;113(2):595-602; discussion 603-4
pubmed: 14758222
Biochem J. 2009 Nov 11;424(2):221-31
pubmed: 19747166
Tissue Eng. 2001 Oct;7(5):573-84
pubmed: 11694191
Cell Motil Cytoskeleton. 2004 Jul;58(3):200-11
pubmed: 15146538
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15229-34
pubmed: 14645704
J Biol Chem. 1999 Jun 18;274(25):17437-44
pubmed: 10364173
Free Radic Biol Med. 2013 Apr;57:68-78
pubmed: 23277143
Acta Pharmacol Sin. 2003 Jul;24(7):637-40
pubmed: 12852827
J Biol Chem. 2006 Aug 18;281(33):23341-8
pubmed: 16735509
Front Physiol. 2021 Feb 26;12:619710
pubmed: 33716768
J Cell Biol. 1997 Jan 13;136(1):137-54
pubmed: 9008709
Microsurgery. 2004;24(4):324-31
pubmed: 15274192
J Cardiovasc Pharmacol. 2004 Oct;44(4):437-45
pubmed: 15454851
Physiol Rev. 2001 Jan;81(1):209-237
pubmed: 11152758
Nature. 1994 Dec 8;372(6506):546-8
pubmed: 7527495
Am J Physiol Cell Physiol. 2008 Apr;294(4):C1088-95
pubmed: 18272817
J Biol Chem. 1997 Feb 21;272(8):4959-63
pubmed: 9030556
Nat Med. 2011 Jun;17(6):720-5
pubmed: 21623381