The effect of iron on the expression levels of calcium related gene in cisplatin resistant epithelial ovarian cancer cells.
Cisplatin
Na+/Ca2+ exchange
calcium
drug resistance
inositol 1,4,5-triphosphate receptor
iron
ryanodine receptor
sarco/endoplasmic reticulum Ca2+ ATPase
Journal
Exploration of targeted anti-tumor therapy
ISSN: 2692-3114
Titre abrégé: Explor Target Antitumor Ther
Pays: United States
ID NLM: 101770662
Informations de publication
Date de publication:
2021
2021
Historique:
received:
28
01
2021
accepted:
14
06
2021
entrez:
1
9
2022
pubmed:
1
1
2021
medline:
1
1
2021
Statut:
ppublish
Résumé
Anticancer drugs (chemotherapeutics) used in cancer treatment (chemotherapy) lead to drug resistance. This study was conducted to investigate the possible effect of iron on calcium homeostasis in epithelial ovarian cancer cells (MDAH-2774) and cisplatin-resistant cells of the same cell line (MDAH-2774/DDP). To develop MDAH-2774/DDP cells, MDAH-2774 (MDAH) cells were treated with cisplatin in dose increases of 5 μM between 0 μM and 70 μM. The effect of iron on the viability of MDAH and MDAH/DDP cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test at the end of 24 h incubation. At increasing iron concentrations in MDAH and MDAH/DDP cells, the mRNA gene of fifteen genes [inositol 1,4,5-triphosphate receptor This changes in the expression of calcium channels, pumps, and exchange proteins in the epithelial ovarian cancer cell line and in cisplatin-resistant epithelial ovarian cancer cells suggest that iron may have an important role in regulating calcium homeostasis. Due to differences in the expression of genes that play of an important role in the regulation of calcium homeostasis in the effect of iron, drug resistance can be prevented by introducing a new perspective on the use of inhibitors and activators of these genes and thus cytostatic treatment strategies.
Identifiants
pubmed: 36046755
doi: 10.37349/etat.2021.00048
pii: etat-02-100248
pmc: PMC9400721
doi:
Types de publication
Journal Article
Langues
eng
Pagination
309-322Informations de copyright
© The Author(s) 2021.
Déclaration de conflit d'intérêts
Regarding this study, the authors and/or family members have no scientific or medical committee membership or relationship with their members, counseling, expertise, employment status in any company, shareholding, and similar situations.
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