A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use.
Candida glabrata
conditional gene expression
mice
virulence
Journal
FEMS yeast research
ISSN: 1567-1364
Titre abrégé: FEMS Yeast Res
Pays: England
ID NLM: 101085384
Informations de publication
Date de publication:
24 09 2022
24 09 2022
Historique:
received:
04
07
2022
revised:
17
08
2022
accepted:
30
08
2022
pubmed:
2
9
2022
medline:
28
9
2022
entrez:
1
9
2022
Statut:
ppublish
Résumé
Candida glabrata is an important pathogen causing superficial to invasive disease in human. Conditional expression systems are helpful in addressing the function of genes and especially when they can be applied to in vivo studies. Tetracycline-dependent regulation systems have been used in diverse fungi to turn-on (Tet-on) or turn-off (Tet-off) gene expression either in vitro but also in vivo in animal models. Up to now, only a Tet-off expression has been constructed for gene expression in C. glabrata. Here, we report a Tet-on gene expression system which can be used in vitro and in vivo in any C. glabrata genetic background. This system was used in a mice model of systemic infection to demonstrate that the general amino acid permease Gap1 is important for C. glabrata virulence.
Identifiants
pubmed: 36047937
pii: 6680246
doi: 10.1093/femsyr/foac046
pmc: PMC9508828
pii:
doi:
Substances chimiques
Amino Acid Transport Systems
0
Tetracycline
F8VB5M810T
Doxycycline
N12000U13O
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of FEMS.
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