Relationships between the expression of adipose genes and profiles of hospitalized dogs.
Adipose tissues
Body condition score
Dogs
Gene expression
Journal
Veterinary research communications
ISSN: 1573-7446
Titre abrégé: Vet Res Commun
Pays: Switzerland
ID NLM: 8100520
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
16
06
2022
accepted:
23
08
2022
pubmed:
2
9
2022
medline:
26
11
2022
entrez:
1
9
2022
Statut:
ppublish
Résumé
Obesity is one of the risk factors for the onset of various metabolic diseases in dogs. Energy expenditure in brown/beige adipocytes, which is partially regulated by the bone morphogenetic protein (BMP) pathway, is a key factor determining systemic energy balance. Here, we examined gene expression in the fat depots of 129 hospitalized dogs, and the relationship between the relative levels of gene expression and profiles of dogs. We evaluated the expression levels of 23 genes such as regulatory genes of adipocyte differentiation and function, adipokines, genes related to brown adipogenesis and uncoupling protein (Ucp), and genes involved in BMP signaling. A reliable equation of multiple regression was not obtained to explain the body condition score (BCS), which is an index of adiposity. Positive relationships were detected between the expression levels of many genes, except for Ucp1 or Ucp3. BCS was found to increase with age. BCS was negatively correlated to the expression levels of Pparγ and Fasn, and positively correlated to Leptin and Opn3 expression. Aging decreased the expression levels of genes related to adipocyte differentiation and function (Pparγ, Fabp4, Fasn, Hsl, and Insr) and Adipoq. In addition, age was negatively correlated with the expression of genes involved in brown adipogenesis and BMP signaling components (Prdm16, Bmp4, Alk3, Actr2a, and Actr2b). In contrast, the expression levels of Leptin and Ucp2 were found to increase with age. The present study clarifies BCS- and age-related gene expressions in the adipose tissue, which potentially contribute to elucidating the etiology of canine obesity.
Identifiants
pubmed: 36048336
doi: 10.1007/s11259-022-09989-2
pii: 10.1007/s11259-022-09989-2
doi:
Substances chimiques
Leptin
0
PPAR gamma
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1239-1244Subventions
Organisme : MEXT-Supported Program for the Private University Research Branding Project
ID : 2016-2020
Organisme : Japan Society for the Promotion of Science
ID : 21K05963
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
Références
Chandler M, Cunningham S, Lund EM, Khanna C, Naramore R, Patel A, Day MJ (2017) Obesity and associated comorbidities in people and companion animals: a one health perspective. J Comp Pathol 156:296–309. https://doi.org/10.1016/j.jcpa.2017.03.006
doi: 10.1016/j.jcpa.2017.03.006
pubmed: 28460795
Chen HJ, Ihara T, Yoshioka H, Itoyama E, Kitamura S, Nagase H, Murakami H, Hoshino Y, Murakami M, Tomonaga S, Matsui T, Funaba M (2018) Expression levels of brown/beige adipocyte-related genes in fat depots of vitamin A-restricted fattening cattle. J Anim Sci 96:3884–3896. https://doi.org/10.1093/jas/sky240
doi: 10.1093/jas/sky240
pubmed: 29912360
pmcid: 6127792
Félix-Soriano E, Sáinz N, Gil-Iturbe E, Collantes M, Fernández-Galilea M, Castilla-Madrigal R, Ly L, Dalli J, Moreno-Aliaga MJ (2021) Changes in brown adipose tissue lipid mediator signatures with aging, obesity, and DHA supplementation in female mice. FASEB J 35:e21592. https://doi.org/10.1096/fj.202002531R
doi: 10.1096/fj.202002531R
pubmed: 33960028
Harms M, Seale P (2013) Brown and beige fat: development, function and therapeutic potential. Nat Med 19:1252–1263. https://doi.org/10.1038/nm.3361
doi: 10.1038/nm.3361
pubmed: 24100998
Jernås M, Palming J, Sjöholm K, Jennische E, Svensson PA, Gabrielsson BG, Levin M, Sjögren A, Rudemo M, Lystig TC, Carlsson B, Carlsson LM, Lönn M (2006) Separation of human adipocytes by size: hypertrophic fat cells display distinct gene expression. FASEB J 20:1540–1542. https://doi.org/10.1096/fj.05-5678fje
doi: 10.1096/fj.05-5678fje
pubmed: 16754744
Kim JB, Spiegelman BM (1996) ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Genes Dev 10:1096–1107. https://doi.org/10.1101/gad.10.9.1096
doi: 10.1101/gad.10.9.1096
pubmed: 8654925
Kontani Y, Wang Y, Kimura K, Inokuma KI, Saito M, Suzuki-Miura T, Wang Z, Sato Y, Mori N, Yamashita H (2005) UCP1 deficiency increases susceptibility to diet-induced obesity with age. Aging Cell 4:147–155. https://doi.org/10.1111/j.1474-9726.2005.00157.x
doi: 10.1111/j.1474-9726.2005.00157.x
pubmed: 15924571
Kubota N, Terauchi Y, Miki H, Tamemoto H, Yamauchi T, Komeda K, Satoh S, Nakano R, Ishii C, Sugiyama T, Eto K, Tsubamoto Y, Okuno A, Murakami K, Sekihara H, Hasegawa G, Naito M, Toyoshima Y, Tanaka S, Shiota K, Kitamura T, Fujita T, Ezaki O, Aizawa S, Nagai R, Tobe K, Kimura S, Kadowaki T (1999) PPARγ mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance. Mol Cell 4:597–609. https://doi.org/10.1016/s1097-2765(00)80210-5
doi: 10.1016/s1097-2765(00)80210-5
pubmed: 10549291
Lavie CJ, Milani RV, Ventura HO (2009) Obesity and cardiovascular disease: risk factor, paradox, and impact of weight loss. J Am Coll Cardiol 53:1925–1932. https://doi.org/10.1016/j.jacc.2008.12.068
doi: 10.1016/j.jacc.2008.12.068
pubmed: 19460605
Loos RJF, Yeo GSH (2022) The genetics of obesity: from discovery to biology. Nat Rev Genet 23:120–133. https://doi.org/10.1038/s41576-021-00414-z
doi: 10.1038/s41576-021-00414-z
pubmed: 34556834
Moerman EJ, Teng K, Lipschitz DA, Lecka-Czernik B (2004) Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells: the role of PPAR-γ2 transcription factor and TGF-β/BMP signaling pathways. Aging Cell 3:379–389. https://doi.org/10.1111/j.1474-9728.2004.00127.x
doi: 10.1111/j.1474-9728.2004.00127.x
pubmed: 15569355
Motomura M, Shimokawa F, Kobayashi T, Yamashita Y, Mizoguchi I, Sato Y, Murakami Y, Shimizu I, Matsui T, Murakami M, Funaba M (2019) Relationships between expression levels of genes related to adipogenesis and adipocyte function in dogs. Mol Biol Rep 46:4771–4777. https://doi.org/10.1007/s11033-019-04923-3
doi: 10.1007/s11033-019-04923-3
pubmed: 31407244
Okamatsu-Ogura Y, Saito M (2021) Brown fat as a regulator of systemic metabolism beyond thermogenesis. Diabetes Metab J 45:840–852. https://doi.org/10.4093/dmj.2020.0291
doi: 10.4093/dmj.2020.0291
Qian SW, Tang Y, Li X, Liu Y, Zhang YY, Huang HY, Xue RD, Yu HY, Guo L, Gao HD, Liu Y, Sun X, Li YM, Jia WP, Tang QQ (2013) BMP4-mediated brown fat-like changes in white adipose tissue alter glucose and energy homeostasis. Proc Natl Acad Sci USA 110:E798–E807. https://doi.org/10.1073/pnas.1215236110
doi: 10.1073/pnas.1215236110
pubmed: 23388637
pmcid: 3587258
Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB (2010) Oxidative stress, inflammation, and cancer: how are they linked? Free Radic Biol Med 49:1603–1616. https://doi.org/10.1016/j.freeradbiomed.2010.09.006
doi: 10.1016/j.freeradbiomed.2010.09.006
pubmed: 20840865
pmcid: 2990475
SAS Institute (2001) SAS User’s Guide: Statistics, Ver. 9.2. SAS Institute, Cary
Sato M, Tsuji T, Yang K, Ren X, Dreyfuss JM, Huang TL, Wang CH, Shamsi F, Leiria LO, Lynes MD, Yau KW, Tseng YH (2020) Cell-autonomous light sensitivity via Opsin3 regulates fuel utilization in brown adipocytes. PLoS Biol 18:e3000630. https://doi.org/10.1371/journal.pbio.3000630
doi: 10.1371/journal.pbio.3000630
pubmed: 32040503
pmcid: 7034924
Seale P, Conroe HM, Estall J, Kajimura S, Frontini A, Ishibashi J, Cohen P, Cinti S, Spiegelman BM (2011) Prdm16 determines the thermogenic program of subcutaneous white adipose tissue in mice. J Clin Invest 121:96–105. https://doi.org/10.1172/JCI44271
doi: 10.1172/JCI44271
pubmed: 21123942
Tseng YH, Kokkotou E, Schulz TJ, Huang TL, Winnay JN, Taniguchi CM, Tran TT, Suzuki R, Espinoza DO, Yamamoto Y, Ahrens MJ, Dudley AT, Norris AW, Kulkarni RN, Kahn CR (2008) New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure. Nature 454:1000–1004. https://doi.org/10.1038/nature07221
doi: 10.1038/nature07221
pubmed: 18719589
pmcid: 2745972
Vidal-Puig AJ, Grujic D, Zhang CY, Hagen T, Boss O, Ido Y, Szczepanik A, Wade J, Mootha V, Cortright R, Muoio DM, Lowell BB (2000) Energy metabolism in uncoupling protein 3 gene knockout mice. J Biol Chem 275:16258–16266. https://doi.org/10.1074/jbc.M910179199
doi: 10.1074/jbc.M910179199
pubmed: 10748196
Yoganathan P, Karunakaran S, Ho MM, Clee SM (2012) Nutritional regulation of genome-wide association obesity genes in a tissue-dependent manner. Nutr Metab (lond) 9:65. https://doi.org/10.1186/1743-7075-9-65
doi: 10.1186/1743-7075-9-65
pubmed: 22781276