The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2.
Journal
bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187
Informations de publication
Date de publication:
25 Aug 2022
25 Aug 2022
Historique:
entrez:
2
9
2022
pubmed:
3
9
2022
medline:
3
9
2022
Statut:
epublish
Résumé
The animal reservoirs of sarbecoviruses represent a significant risk of emergent pandemics, as evidenced by the impact of SARS-CoV-2. Vaccines remain successful at limiting severe disease and death, however the continued emergence of SARS-CoV-2 variants, together with the potential for further coronavirus zoonosis, motivates the search for pan-coronavirus vaccines that induce broadly neutralizing antibodies. This necessitates a better understanding of the glycan shields of coronaviruses, which can occlude potential antibody epitopes on spike glycoproteins. Here, we compare the structure of several sarbecovirus glycan shields. Many N-linked glycan attachment sites are shared by all sarbecoviruses, and the processing state of certain sites is highly conserved. However, there are significant differences in the processing state at several glycan sites that surround the receptor binding domain. Our studies reveal similarities and differences in the glycosylation of sarbecoviruses and show how subtle changes in the protein sequence can have pronounced impacts on the glycan shield.
Identifiants
pubmed: 36052375
doi: 10.1101/2022.08.24.505118
pmc: PMC9435400
pii:
doi:
Types de publication
Preprint
Langues
eng
Subventions
Organisme : Bill & Melinda Gates Foundation
ID : INV-004923
Pays : United States
Organisme : Bill & Melinda Gates Foundation
ID : INV-008352
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI144462
Pays : United States
Commentaires et corrections
Type : UpdateIn
Références
Nature. 2020 Aug;584(7819):120-124
pubmed: 32454512
Sci Rep. 2017 Sep 5;7(1):10480
pubmed: 28874689
Biochim Biophys Acta Gen Subj. 2019 Oct;1863(10):1480-1497
pubmed: 31121217
Science. 2020 Sep 18;369(6510):1501-1505
pubmed: 32703906
Nucleic Acids Res. 2017 Jan 4;45(D1):D170-D176
pubmed: 27899574
Nat Struct Mol Biol. 2016 Oct;23(10):899-905
pubmed: 27617430
Bioinformatics. 2020 Mar 1;36(6):1765-1771
pubmed: 31697312
EBioMedicine. 2022 Apr;78:103944
pubmed: 35465948
Nature. 2020 Aug;584(7821):437-442
pubmed: 32555388
BMC Infect Dis. 2022 May 7;22(1):439
pubmed: 35525973
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
Science. 2020 Aug 28;369(6507):1119-1123
pubmed: 32661058
J Mol Biol. 2022 Jan 30;434(2):167332
pubmed: 34717971
PLoS Comput Biol. 2021 Jan 28;17(1):e1008667
pubmed: 33507980
J Virol. 2017 Jan 3;91(2):
pubmed: 27807235
Cell. 2020 Jul 9;182(1):73-84.e16
pubmed: 32425270
BMC Bioinformatics. 2019 Sep 14;20(1):473
pubmed: 31521110
Nature. 2020 Jul;583(7815):290-295
pubmed: 32422645
Protein Sci. 2011 Jan;20(1):179-86
pubmed: 21082725
Science. 2021 Feb 12;371(6530):735-741
pubmed: 33436524
Nat Med. 2020 Apr;26(4):450-452
pubmed: 32284615
Science. 2020 Aug 7;369(6504):650-655
pubmed: 32571838
Acta Pharmacol Sin. 2020 Sep;41(9):1141-1149
pubmed: 32747721
Clin Med (Lond). 2004 Mar-Apr;4(2):152-60
pubmed: 15139736
Cell. 2020 Apr 16;181(2):281-292.e6
pubmed: 32155444
Commun Biol. 2022 Aug 4;5(1):785
pubmed: 35927436
Nature. 2013 Nov 28;503(7477):535-8
pubmed: 24172901
Cell Host Microbe. 2021 Mar 10;29(3):327-333
pubmed: 33705704
ACS Cent Sci. 2021 Apr 28;7(4):586-593
pubmed: 34056088
Nat Commun. 2017 Mar 28;8:14954
pubmed: 28348411
Microbiol Resour Announc. 2019 Jul 11;8(28):
pubmed: 31296683
J Virol. 2002 Jul;76(14):7306-21
pubmed: 12072529
Emerg Microbes Infect. 2020 Dec;9(1):1567-1579
pubmed: 32602823
Commun Biol. 2022 Apr 11;5(1):342
pubmed: 35411021
Nature. 2020 Aug;584(7819):115-119
pubmed: 32454513
PLoS Pathog. 2021 Mar 22;17(3):e1009407
pubmed: 33750987
Nat Commun. 2021 Mar 17;12(1):1715
pubmed: 33731724
Cell Rep. 2021 Sep 28;36(13):109760
pubmed: 34534459
Nature. 2020 Jul;583(7815):282-285
pubmed: 32218527
Science. 2020 Aug 21;369(6506):956-963
pubmed: 32540903
Nat Microbiol. 2020 Apr;5(4):562-569
pubmed: 32094589
J Mol Med (Berl). 2021 Aug;99(8):1023-1031
pubmed: 34023935
J Mol Biol. 2019 May 31;431(12):2223-2247
pubmed: 31028779
Cell Rep. 2021 Apr 6;35(1):108933
pubmed: 33826885
Science. 2020 Jun 12;368(6496):1274-1278
pubmed: 32404477
Science. 2020 Sep 18;369(6510):1505-1509
pubmed: 32703908
Nat Rev Nephrol. 2019 Jun;15(6):346-366
pubmed: 30858582
Nat Commun. 2020 May 27;11(1):2688
pubmed: 32461612
Science. 2019 Feb 8;363(6427):649-654
pubmed: 30573546
Cell. 2021 May 27;184(11):2955-2972.e25
pubmed: 34019795
Nat Struct Mol Biol. 2020 Oct;27(10):925-933
pubmed: 32699321
Nat Rev Microbiol. 2019 Mar;17(3):181-192
pubmed: 30531947
Nucleic Acids Res. 2017 Jan 4;45(D1):D313-D319
pubmed: 27899672
Nucleic Acids Res. 2018 Jul 2;46(W1):W296-W303
pubmed: 29788355
Biochemistry. 2021 Jul 13;60(27):2153-2169
pubmed: 34213308
Trends Microbiol. 2007 May;15(5):211-8
pubmed: 17398101
Science. 2020 Jul 17;369(6501):330-333
pubmed: 32366695
Cell Rep. 2018 Aug 21;24(8):1958-1966.e5
pubmed: 30134158
Cell Rep. 2015 Jun 16;11(10):1604-13
pubmed: 26051934
Proc Natl Acad Sci U S A. 2018 Jul 10;115(28):7320-7325
pubmed: 29941589
ACS Cent Sci. 2020 Oct 28;6(10):1722-1734
pubmed: 33140034
Front Immunol. 2021 Mar 09;12:637651
pubmed: 33767706
Nat Immunol. 2022 Jun;23(6):960-970
pubmed: 35654851
Immunity. 2020 Jul 14;53(1):98-105.e5
pubmed: 32561270
Cell Host Microbe. 2020 Oct 7;28(4):586-601.e6
pubmed: 32841605
Curr Biol. 2020 Jun 8;30(11):2196-2203.e3
pubmed: 32416074
JAMA. 2021 Mar 2;325(9):821-822
pubmed: 33507218
Cell. 2020 Aug 20;182(4):828-842.e16
pubmed: 32645326
Curr Opin Struct Biol. 2017 Jun;44:125-133
pubmed: 28363124
Germs. 2019 Mar 01;9(1):35-42
pubmed: 31119115
BMC Bioinformatics. 2009 Dec 15;10:421
pubmed: 20003500
Nature. 2020 May;581(7808):252-255
pubmed: 32415276
Life Sci Alliance. 2020 Jul 23;3(9):
pubmed: 32703818
Glycobiology. 2017 Jan;27(1):3-49
pubmed: 27558841