Identification and analysis of B cell epitopes of hemagglutinin of H1N1 influenza virus.

Animals Antibodies, Monoclonal Antibodies, Viral Computer Simulation Epitopes, B-Lymphocyte Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins Influenza A Virus, H1N1 Subtype Mice Mice, Inbred BALB C
Computer prediction ELISA blocking test H1N1 influenza virus Monoclonal antibody (mAb)

Journal

Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427

Informations de publication

Date de publication:
02 Sep 2022
Historique:
received: 19 05 2022
accepted: 10 07 2022
revised: 06 07 2022
entrez: 2 9 2022
pubmed: 3 9 2022
medline: 8 9 2022
Statut: epublish

Résumé

The frequent variation of influenza virus hemagglutinin (HA) antigen is the main cause of influenza pandemic. Therefore, the study of B cell epitopes of HA is of great significance in the prevention and control of influenza virus. In this study, the split vaccine of 2009 H1N1 influenza virus was used as immunogen, and the monoclonal antibodies (mAbs) were prepared by conventional hybridoma fusion and screening techniques. The characteristics of mAbs were identified by ELISA method, Western-blot test and hemagglutination inhibition test (HI). Using the obtained mAbs as a tool, the B cell epitopes of HA were predicted by ELISA blocking test, sandwich ELISA method and computer simulation method. Finally, four mAbs against HA antigen of H1N1 influenza virus were obtained. The results of ELISA and computer prediction showed that there were at least two types of epitopes on HA of influenza virus. The results of this study complemented the existing methods for predicting HA epitopes, and also provided a new method for predicting other pathogenic microorganisms.

Identifiants

DOI: 10.1007/s00203-022-03133-z PMID: 36053375 PMC: PMC9438888
pubmed: 36053375
doi: 10.1007/s00203-022-03133-z
pii: 10.1007/s00203-022-03133-z
pmc: PMC9438888
doi:

Substances chimiques

Antibodies, Monoclonal 0
Antibodies, Viral 0
Epitopes, B-Lymphocyte 0
Hemagglutinin Glycoproteins, Influenza Virus 0
Hemagglutinins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

594

Subventions

Organisme : Key Research and Development Program of Shaanxi
ID : 2021ZDLSF01-03
Organisme : Incubation Fund Program of Shaanxi Provincial People's Hospital
ID : 2021YJY-27

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Références

PLoS Pathog. 2022 Jan 6;18(1):e1010169
pubmed: 34990480
J Virol. 2014 Nov;88(21):12364-73
pubmed: 25122788
Microb Pathog. 2020 Aug;145:104236
pubmed: 32376359
Sci Rep. 2018 Aug 7;8(1):11856
pubmed: 30087372
Appl Soft Comput. 2022 Feb;116:108280
pubmed: 34931117
Cell. 2021 Dec 9;184(25):6052-6066.e18
pubmed: 34852239
J Med Virol. 2022 Jun;94(6):2558-2567
pubmed: 35005794
BMC Vet Res. 2018 Dec 22;14(1):415
pubmed: 30577861
Clin Infect Dis. 2019 Oct 30;69(10):1817-1823
pubmed: 31102401
PLoS One. 2018 Dec 21;13(12):e0207777
pubmed: 30576319
PLoS One. 2021 Nov 15;16(11):e0258645
pubmed: 34780495
Virology. 2015 Nov;485:252-62
pubmed: 26318247
Nature. 2022 Apr;604(7906):553-556
pubmed: 35240676
Clin Microbiol Rev. 2013 Jul;26(3):476-92
pubmed: 23824369
Exp Mol Pathol. 2015 Feb;98(1):79-84
pubmed: 25533547
Vaccine. 2011 Jul 12;29(31):4897-902
pubmed: 21596084
PLoS One. 2010 Jul 12;5(7):e11548
pubmed: 20634959
Science. 2020 May 8;368(6491):630-633
pubmed: 32245784
Virol Sin. 2019 Jun;34(3):306-314
pubmed: 31020574
Anal Chim Acta. 2020 May 1;1109:148-157
pubmed: 32252898
Plant J. 2021 May;106(4):993-1007
pubmed: 33629439
J Clin Invest. 2019 Jan 2;129(1):93-105
pubmed: 30457979
J Virol. 2019 Feb 5;93(4):
pubmed: 30518651
Antiviral Res. 2021 Feb;186:105013
pubmed: 33428962
J Clin Invest. 2018 Nov 1;128(11):4992-4996
pubmed: 30188868
PeerJ. 2021 Dec 20;9:e12512
pubmed: 35036116
J Mater Chem B. 2022 Sep 15;10(35):6644-6654
pubmed: 35502890
Viruses. 2021 Oct 21;13(11):
pubmed: 34834932
Nature. 2015 Jul 9;523(7559):217-20
pubmed: 26053121
Biochemistry (Mosc). 2021 Nov;86(11):1469-1476
pubmed: 34906050
Science. 2020 May 15;368(6492):
pubmed: 32409444
J Clin Invest. 2021 Aug 2;131(15):
pubmed: 34156974
mBio. 2021 Jun 29;12(3):e0114421
pubmed: 34060327
Emerg Microbes Infect. 2018 May 31;7(1):100
pubmed: 29855467

Auteurs

Qing Feng (Q)

Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, Shaanxi, China.
Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Xi'an, Shaanxi, China.
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi, China.

Xiao-Yan Huang (XY)

Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Xi'an, Shaanxi, China.

Yang-Meng Feng (YM)

Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, Shaanxi, China.
Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Xi'an, Shaanxi, China.
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi, China.

Li-Jun Sun (LJ)

Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, Shaanxi, China.
Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Xi'an, Shaanxi, China.
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi, China.

Jing-Ying Sun (JY)

Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, Shaanxi, China.
Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Xi'an, Shaanxi, China.
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi, China.

Yan Li (Y)

Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, Shaanxi, China.
Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Xi'an, Shaanxi, China.
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi, China.

Xin Xie (X)

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, China.

Jun Hu (J)

Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, Shaanxi, China. hjj65622@163.com.
Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Xi'an, Shaanxi, China. hjj65622@163.com.
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi, China. hjj65622@163.com.

Chun-Yan Guo (CY)

Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, 710068, Shaanxi, China. guochunyan101109@126.com.
Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Xi'an, Shaanxi, China. guochunyan101109@126.com.
Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi, China. guochunyan101109@126.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Identification and analysis of B cell epitopes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Identification and analysis of B cell epitopes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antiviraux › Identification and analysis of B cell epitopes...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Identification and analysis of B cell epitopes...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Épitopes › Déterminants antigéniques des lymphocytes B › Identification and analysis of B cell epitopes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines de l'enveloppe virale › Glycoprotéine hémagglutinine du virus influenza › Identification and analysis of B cell epitopes...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Agents hématologiques › Coagulants › Agglutinines › Hémagglutinines › Identification and analysis of B cell epitopes...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN de polarité négative › Orthomyxoviridae › Influenzavirus A › Virus de la grippe A › Sous-type H1N1 du virus de la grippe A › Identification and analysis of B cell epitopes...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Identification and analysis of B cell epitopes...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée BALB C › Identification and analysis of B cell epitopes...