Developing transmission mode for infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging.
Journal
Rapid communications in mass spectrometry : RCM
ISSN: 1097-0231
Titre abrégé: Rapid Commun Mass Spectrom
Pays: England
ID NLM: 8802365
Informations de publication
Date de publication:
30 Nov 2022
30 Nov 2022
Historique:
revised:
27
08
2022
received:
20
07
2022
accepted:
27
08
2022
pubmed:
4
9
2022
medline:
12
10
2022
entrez:
3
9
2022
Statut:
ppublish
Résumé
The development and characterization of the novel NextGen infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) source catalyzed new advancements in IR-MALDESI instrumentation, including the development of a new analysis geometry. A vertically oriented transmission mode (tm)-IR-MALDESI setup was developed and optimized on thawed mouse tissue. In addition, glycerol was introduced as an alternative energy-absorbing matrix for tm-IR-MALDESI because the new geometry does not currently allow for the formation of an ice matrix. The tm geom was evaluated against the optimized standard geometry for the NextGen source in reflection mode (rm). It was found that tm-IR-MALDESI produces comparable results to rm-IR-MALDESI after optimization. The attempt to incorporate glycerol as an alternative matrix provided little improvement to tm-IR-MALDESI ion abundances. This work has successfully demonstrated the adaptation of the NextGen IR-MALDESI source through the feasibility of tm-IR-MALDESI mass spectrometry imaging on mammalian tissue, expanding future biological applications of the method.
Identifiants
pubmed: 36056474
doi: 10.1002/rcm.9386
pmc: PMC9541130
mid: NIHMS1834340
doi:
Substances chimiques
Ice
0
Glycerol
PDC6A3C0OX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e9386Subventions
Organisme : North Carolina State University
Organisme : NIGMS NIH HHS
ID : T32 GM133366
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM087964
Pays : United States
Organisme : NIH Molecular Biotechnology Training Program
ID : 5T32GM133366-03
Organisme : National Institute of Health
ID : R01GM087964
Informations de copyright
© 2022 The Authors. Rapid Communications in Mass Spectrometry published by John Wiley & Sons Ltd.
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