Biofilms with a Dash of Color: A Hands-On Activity for School Students to Build a Biofilm Model and Use It to Understand Antibiotic Tolerance in Biofilms.
antibiotic tolerance
antibiotics
bacteria
biofilms
extracellular matrix
free-living bacteria
hands-on
hands-on activity
matrix
microbes
tolerance
Journal
Journal of microbiology & biology education
ISSN: 1935-7877
Titre abrégé: J Microbiol Biol Educ
Pays: United States
ID NLM: 101543341
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
received:
11
07
2022
accepted:
24
07
2022
entrez:
5
9
2022
pubmed:
6
9
2022
medline:
6
9
2022
Statut:
epublish
Résumé
It is increasingly recognized that microbes such as bacteria exist in communities or biofilms, both in the environment and human body. However, school biology curricula continue to focus on the free-floating form of bacterial life, with minimal descriptions of biofilms. Consequently, there is a need to introduce biofilms to school students, to not only to develop a fundamental understanding of microbial life but also to highlight the challenges posed by biofilm infections to antibiotic treatment. We have developed a hands-on activity for students to build a biofilm model and use it in comparison with a model of free-living (planktonic) bacteria, to test the role of the extracellular matrix in the antibiotic tolerance of biofilms. The activity uses simple, easy-to-obtain supplies and is designed to be conducted in an in-person or virtual format for elementary and middle school students in the age group of 6 to 13 years. We conducted the activity in virtual mode (via Zoom) for 59 school students across India, and we present feedback and acquired knowledge that could be used to execute and adapt this accessible and engaging science experience.
Identifiants
pubmed: 36061327
doi: 10.1128/jmbe.00099-22
pii: 00099-22
pmc: PMC9429959
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright © 2022 Kadam et al.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Annu Rev Microbiol. 1995;49:711-45
pubmed: 8561477
Int J Med Microbiol. 2002 Jul;292(2):107-13
pubmed: 12195733
J Bacteriol. 2000 May;182(10):2675-9
pubmed: 10781532
J Microbiol Biol Educ. 2017 Apr 21;18(1):
pubmed: 28512515
J Microbiol Biol Educ. 2013 Dec 02;14(2):252-4
pubmed: 24358392
J Microbiol Biol Educ. 2018 Apr 27;19(1):
pubmed: 29904559
Science. 1999 May 21;284(5418):1318-22
pubmed: 10334980
Microbiol Spectr. 2015 Jun;3(3):
pubmed: 26185072