Stem Cell Exosomes Improve Survival of Neural Stem Cells after Radiation Exposure.
cell therapy
exosomes
gamma radiation
mesenchymal stem cells
neural stem cells
Journal
Bulletin of experimental biology and medicine
ISSN: 1573-8221
Titre abrégé: Bull Exp Biol Med
Pays: United States
ID NLM: 0372557
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
received:
03
03
2022
pubmed:
6
9
2022
medline:
24
9
2022
entrez:
5
9
2022
Statut:
ppublish
Résumé
The death of neural stem cells in the hippocampus during radiation therapy of brain tumors leads to neurogenesis impairment and the development of cognitive dysfunctions at delayed terms after irradiation. Exosomes secreted by stem cells can provide a protective effect on neural stem cells. We isolated and characterized exosomes from the medium conditioned by neural stem cells and mesenchymal stem cells from mouse adipose tissue and studied their efficiency in protecting irradiated neural stem cells. According to dynamic light scattering data, the exosome size varied from 44 to 68 nm for neural stem cells, and from 80 to 130 nm for mesenchymal stem cells. All exosomes carried markers CD9, CD63, and TSG101. The survival rate and clonogenic activity of neural stem cells irradiated in a dose of 1 Gy was found to increase after culturing in the presence of stem cell exosome preparations.
Identifiants
pubmed: 36063301
doi: 10.1007/s10517-022-05587-z
pii: 10.1007/s10517-022-05587-z
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
544-552Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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