Associations between polymorphisms of LAP3 and SIRT1 genes with clinical mastitis and milk production traits in Sahiwal and Karan Fries dairy cattle.
SNP
association
clinical mastitis
dairy cattle
milk production traits
Journal
Veterinary medicine and science
ISSN: 2053-1095
Titre abrégé: Vet Med Sci
Pays: England
ID NLM: 101678837
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
pubmed:
6
9
2022
medline:
23
11
2022
entrez:
5
9
2022
Statut:
ppublish
Résumé
Bovine mastitis continues to remain as the most challenging disease in dairy cattle, as a result improvement of selection methods has great economic relevance while a deeper understanding of the genetic mechanisms regulating milk production traits and mastitis is of general scientific interest. This study aimed to evaluate the association of SNPs of the LAP3 and SIRT1 genes with estimated breeding values (EBVs) of milk production traits and clinical mastitis in dairy cattle of Indian origin. DNA samples from 263 animals (Sahiwal and Karan Fries cattle) were genotyped by PCR-RFLP to assess their pattern of genetic variation. EBVs of milk production traits and phenotypic records of incidences of clinical mastitis were used for association analysis. A total of 9 SNPs were identified, including three (rs110932626: A>G, rs716493845: C>T and rs43702363: C>T) in intron 12, four (g.24904G>C, rs110839532: G>T, rs43702361: T>C and rs41255599: C>T) in exon 13 and within 3'UTR of LAP3 gene and two (rs110250233: G>A and rs42140046: C>G) in the promoter region of SIRT1 gene. Eight of these identified SNPs were chosen for subsequent genotyping and association analyses. Association analysis revealed that SNP rs41255599: C>T was significantly associated with lactation milk yield, 305-day milk yield, 305-day fat yield, 305-day solid not fat yield, lactation length and incidence of clinical mastitis (p < 0.05) in Sahiwal cattle. For Karan Fries cattle, two SNPs including rs110932626: A>G and rs43702363: C>T showed significant association with 305-day milk yield. Overall, these findings provide evidence for association of the LAP3 gene with milk production traits and clinical mastitis in dairy cattle, indicating the potential role of LAP3 variants in these traits.
Sections du résumé
BACKGROUND
Bovine mastitis continues to remain as the most challenging disease in dairy cattle, as a result improvement of selection methods has great economic relevance while a deeper understanding of the genetic mechanisms regulating milk production traits and mastitis is of general scientific interest.
OBJECTIVES
This study aimed to evaluate the association of SNPs of the LAP3 and SIRT1 genes with estimated breeding values (EBVs) of milk production traits and clinical mastitis in dairy cattle of Indian origin.
METHODS
DNA samples from 263 animals (Sahiwal and Karan Fries cattle) were genotyped by PCR-RFLP to assess their pattern of genetic variation. EBVs of milk production traits and phenotypic records of incidences of clinical mastitis were used for association analysis.
RESULTS
A total of 9 SNPs were identified, including three (rs110932626: A>G, rs716493845: C>T and rs43702363: C>T) in intron 12, four (g.24904G>C, rs110839532: G>T, rs43702361: T>C and rs41255599: C>T) in exon 13 and within 3'UTR of LAP3 gene and two (rs110250233: G>A and rs42140046: C>G) in the promoter region of SIRT1 gene. Eight of these identified SNPs were chosen for subsequent genotyping and association analyses. Association analysis revealed that SNP rs41255599: C>T was significantly associated with lactation milk yield, 305-day milk yield, 305-day fat yield, 305-day solid not fat yield, lactation length and incidence of clinical mastitis (p < 0.05) in Sahiwal cattle. For Karan Fries cattle, two SNPs including rs110932626: A>G and rs43702363: C>T showed significant association with 305-day milk yield.
CONCLUSION
Overall, these findings provide evidence for association of the LAP3 gene with milk production traits and clinical mastitis in dairy cattle, indicating the potential role of LAP3 variants in these traits.
Identifiants
pubmed: 36063537
doi: 10.1002/vms3.924
pmc: PMC9677372
doi:
Substances chimiques
Sirtuin 1
EC 3.5.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2593-2604Informations de copyright
© 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
Références
Anim Genet. 2009 Aug;40(4):492-8
pubmed: 19392827
DNA Cell Biol. 2012 Jun;31(6):1038-45
pubmed: 22304649
Sci Rep. 2017 Mar 30;7:45560
pubmed: 28358110
Anim Genet. 2018 Apr;49(2):103-109
pubmed: 29368428
J Anim Sci. 2011 Aug;89(8):2529-36
pubmed: 21421831
Arch Anim Breed. 2019 Mar 22;62(1):107-112
pubmed: 31807620
Anim Genet. 2013 Aug;44(5):584-7
pubmed: 23647079
Anim Genet. 2009 Dec;40(6):832-51
pubmed: 19508288
Genetics. 2005 Jan;169(1):275-83
pubmed: 15466433
Int J Mol Sci. 2015 Jan 22;16(2):2458-71
pubmed: 25622258
Genet Sel Evol. 2020 Oct 1;52(1):55
pubmed: 32998688
Mol Biol Rep. 2011 Aug;38(6):4053-61
pubmed: 21110109
Vet Clin North Am Food Anim Pract. 2018 Nov;34(3):457-472
pubmed: 30316503
Science. 2004 Jul 16;305(5682):390-2
pubmed: 15205477
Biol Chem. 2006 Dec;387(12):1535-44
pubmed: 17132098
J Mammary Gland Biol Neoplasia. 2011 Dec;16(4):291-304
pubmed: 21938490
Genet Sel Evol. 2004 Mar-Apr;36(2):163-90
pubmed: 15040897
Curr Opin Immunol. 2004 Feb;16(1):76-81
pubmed: 14734113
Yi Chuan. 2003 May;25(3):327-9
pubmed: 15639881
J Dairy Sci. 2008 Mar;91(3):1189-97
pubmed: 18292275
PLoS One. 2012;7(12):e50137
pubmed: 23226511
Mol Cell Probes. 2013 Oct-Dec;27(5-6):215-20
pubmed: 23871946
Front Genet. 2021 Mar 22;12:643531
pubmed: 33828586
Vet Med Sci. 2022 Nov;8(6):2593-2604
pubmed: 36063537
Animal. 2019 Oct;13(10):2156-2163
pubmed: 30835192
J Dairy Sci. 2014 Nov;97(11):7258-75
pubmed: 25151887
Genome Res. 2005 Jul;15(7):936-44
pubmed: 15998908
Gene. 2019 Jun 5;700:47-51
pubmed: 30902782