Biochemical, genomic, and epigenomic profiling of isolated cancer cell lines' micronuclei.
ATAC-Seq
Chromosomal instability
Histone mass spectrometry
Mass spectrometry
Micronuclei isolation
Whole genome sequencing
Whole-genome bisulfite sequencing
Journal
Methods in cell biology
ISSN: 0091-679X
Titre abrégé: Methods Cell Biol
Pays: United States
ID NLM: 0373334
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
5
9
2022
pubmed:
6
9
2022
medline:
9
9
2022
Statut:
ppublish
Résumé
Micronuclei are common byproducts of chromosomally unstable cancer cells during their division. Micronuclei play an important role in cancer metastasis as well as introducing chromosomal abnormalities into the primary nucleus in the subsequent cell cycle. Given their major role in tumor initiation and progression, methods to examine their properties are crucially needed. This chapter discusses approaches and methods to profile micronuclei's biochemical, genomic, and epigenomic properties following their physical isolation. Using either MPS1 inhibition or radiation to induce formation of micronuclei, this method introduces a versatile way to investigate biological events that occur in micronuclei as well as compare them to events in the primary nuclei from the same system.
Identifiants
pubmed: 36064226
pii: S0091-679X(22)00092-9
doi: 10.1016/bs.mcb.2022.07.001
pii:
doi:
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
51-66Subventions
Organisme : NIH HHS
ID : DP5 OD026395
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA247749
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA256188
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Disclosures SFB owns equity in, receives compensation from, and serves as a consultant and the Scientific Advisory Board and Board of Directors of Volastra Therapeutics Inc. AA declares no conflict of interest.