CD8 memory precursor cell generation is a continuous process.
Bioinformatics
Cell
Cell biology
Immunology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
16 Sep 2022
16 Sep 2022
Historique:
received:
09
03
2022
revised:
21
06
2022
accepted:
09
08
2022
entrez:
6
9
2022
pubmed:
7
9
2022
medline:
7
9
2022
Statut:
epublish
Résumé
In this work, we studied the generation of memory precursor cells following an acute infection by analyzing single-cell RNA-seq data that contained CD8 T cells collected during the postinfection expansion phase. We used different tools to reconstruct the developmental trajectory that CD8 T cells followed after activation. Cells that exhibited a memory precursor signature were identified and positioned on this trajectory. We found that these memory precursors are generated continuously with increasing numbers arising over time. Similarly, expression of genes associated with effector functions was also found to be raised in memory precursors at later time points. The ability of cells to enter quiescence and differentiate into memory cells was confirmed by BrdU pulse-chase experiment
Identifiants
pubmed: 36065187
doi: 10.1016/j.isci.2022.104927
pii: S2589-0042(22)01199-3
pmc: PMC9440290
doi:
Types de publication
Journal Article
Langues
eng
Pagination
104927Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Nat Immunol. 2019 Feb;20(2):163-172
pubmed: 30643263
Science. 2007 Mar 23;315(5819):1687-91
pubmed: 17332376
Immunity. 2020 Nov 17;53(5):985-1000.e11
pubmed: 33128876
Immunity. 2007 Aug;27(2):281-95
pubmed: 17723218
Cell. 2021 Jun 24;184(13):3573-3587.e29
pubmed: 34062119
F1000Res. 2016 Aug 31;5:2122
pubmed: 27909575
Nat Rev Immunol. 2018 May;18(5):340-356
pubmed: 29379213
Nat Immunol. 2015 Sep;16(9):891-3
pubmed: 26287584
Nat Immunol. 2014 Apr;15(4):365-372
pubmed: 24584088
BMC Genomics. 2018 Jun 19;19(1):477
pubmed: 29914354
Science. 2001 Mar 23;291(5512):2413-7
pubmed: 11264538
Cell Syst. 2017 Mar 22;4(3):306-317.e4
pubmed: 28237797
Sci Immunol. 2020 May 15;5(47):
pubmed: 32414833
Nat Biotechnol. 2020 Dec;38(12):1408-1414
pubmed: 32747759
Nat Immunol. 2022 May;23(5):791-801
pubmed: 35393592
Immunity. 2016 Apr 19;44(4):755-68
pubmed: 26992565
Immunity. 2010 Aug 27;33(2):229-40
pubmed: 20727791
Immunity. 2019 Nov 19;51(5):840-855.e5
pubmed: 31606264
Nat Rev Cancer. 2012 Oct;12(10):671-84
pubmed: 22996603
Nature. 1999 Oct 14;401(6754):708-12
pubmed: 10537110
Nat Immunol. 2011 Nov 06;12(12):1221-9
pubmed: 22057289
Proc Natl Acad Sci U S A. 2010 May 25;107(21):9777-82
pubmed: 20457902
Nature. 1999 Jun 10;399(6736):593-7
pubmed: 10376601
PLoS Pathog. 2017 Aug 21;13(8):e1006544
pubmed: 28827827
J Immunol. 2018 May 15;200(10):3635-3646
pubmed: 29632146
Bioinformatics. 2020 Jul 1;36(Suppl_1):i66-i74
pubmed: 32657409
Nat Biotechnol. 2019 May;37(5):547-554
pubmed: 30936559
J Exp Med. 2009 Feb 16;206(2):435-48
pubmed: 19188498
Nat Immunol. 2005 Dec;6(12):1236-44
pubmed: 16273099
Immunol Res. 2012 Jun;52(3):284-93
pubmed: 22528127
Genome Biol. 2019 Dec 23;20(1):296
pubmed: 31870423
J Immunol. 1999 May 1;162(9):5053-7
pubmed: 10227972
J Exp Med. 2015 Nov 16;212(12):2027-39
pubmed: 26503445
J Exp Med. 2007 Jul 9;204(7):1625-36
pubmed: 17606632
Immunity. 2017 Apr 18;46(4):596-608
pubmed: 28410989
Nat Methods. 2017 Nov;14(11):1083-1086
pubmed: 28991892
Nat Rev Immunol. 2012 Nov;12(11):749-61
pubmed: 23080391
Immunity. 1998 Feb;8(2):177-87
pubmed: 9491999
J Exp Med. 2005 Feb 21;201(4):579-90
pubmed: 15710650
Science. 2013 May 3;340(6132):635-9
pubmed: 23493421
Immunity. 2007 Oct;27(4):670-84
pubmed: 17950003
Science. 2013 May 3;340(6132):630-5
pubmed: 23493420
J Immunol. 2006 Oct 1;177(7):4451-7
pubmed: 16982880
J Exp Med. 2018 Mar 5;215(3):773-783
pubmed: 29440362
J Virol. 2003 Apr;77(8):4911-27
pubmed: 12663797
Nat Immunol. 2019 Sep;20(9):1092-1094
pubmed: 31427776
J Exp Med. 2008 Mar 17;205(3):625-40
pubmed: 18316415
Immunity. 2009 Dec 18;31(6):859-71
pubmed: 20064446
Bioinformatics. 2017 Apr 15;33(8):1179-1186
pubmed: 28088763
Eur J Immunol. 2016 Jul;46(7):1548-62
pubmed: 27230488
Nat Med. 2002 Apr;8(4):379-85
pubmed: 11927944
Nat Immunol. 2019 Jul;20(7):890-901
pubmed: 31209400
Science. 2018 Jan 12;359(6372):177-186
pubmed: 29326266
Sci Immunol. 2021 Jan 15;6(55):
pubmed: 33452106
Nat Biotechnol. 2021 Jul;39(7):813-818
pubmed: 33795888
Nat Immunol. 2017 Apr;18(4):422-432
pubmed: 28218746
Science. 2016 Apr 8;352(6282):189-96
pubmed: 27124452
Immunity. 2010 Jan 29;32(1):91-103
pubmed: 20096608
Nat Commun. 2020 Jan 8;11(1):113
pubmed: 31913278
Immunity. 2014 Jul 17;41(1):116-26
pubmed: 25035956
Nature. 2017 Dec 21;552(7685):404-409
pubmed: 29236683
Nat Rev Immunol. 2018 Jan;18(1):35-45
pubmed: 28787399
Nat Commun. 2015 Feb 24;6:6301
pubmed: 25709008
Immunity. 2008 Jun;28(6):859-69
pubmed: 18499487
PLoS Biol. 2017 Jul 27;15(7):e2001867
pubmed: 28749943