The cytoprotective sequestration activity of small heat shock proteins is evolutionarily conserved.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
03 10 2022
03 10 2022
Historique:
received:
28
02
2022
revised:
21
07
2022
accepted:
16
08
2022
entrez:
7
9
2022
pubmed:
8
9
2022
medline:
11
9
2022
Statut:
ppublish
Résumé
The chaperone-mediated sequestration of misfolded proteins into inclusions is a pivotal cellular strategy to maintain proteostasis in Saccharomyces cerevisiae, executed by small heat shock proteins (sHsps) Hsp42 and Btn2. Direct homologs of Hsp42 and Btn2 are absent in other organisms, questioning whether sequestration represents a conserved proteostasis strategy and, if so, which factors are involved. We examined sHsps from Escherchia coli, Caenorhabditis elegans, and humans for their ability to complement the defects of yeast sequestrase mutants. We show that sequestration of misfolded proteins is an original and widespread activity among sHsps executed by specific family members. Sequestrase positive C. elegans' sHsps harbor specific sequence features, including a high content of aromatic and methionine residues in disordered N-terminal extensions. Those sHsps buffer limitations in Hsp70 capacity in C. elegans WT animals and are upregulated in long-lived daf-2 mutants, contributing to lifespan extension. Cellular protection by sequestration of misfolded proteins is, therefore, an evolutionarily conserved activity of the sHsp family.
Identifiants
pubmed: 36069810
pii: 213447
doi: 10.1083/jcb.202202149
pmc: PMC9458469
pii:
doi:
Substances chimiques
HSP42 protein, S cerevisiae
0
Heat-Shock Proteins, Small
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
Organisme : German Research Foundation
ID : SFB1036
Organisme : Helmholtz Zukunftsthema Aging
ID : ZT-0026
Informations de copyright
© 2022 Shrivastava et al.
Références
FASEB J. 2010 Oct;24(10):3633-42
pubmed: 20501794
Cell. 2012 Apr 13;149(2):274-93
pubmed: 22500797
Cell Rep. 2016 Aug 9;16(6):1717-1732
pubmed: 27477278
Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14914-9
pubmed: 19706382
J Biol Chem. 2019 Feb 8;294(6):2121-2132
pubmed: 30385502
Genetics. 1974 May;77(1):71-94
pubmed: 4366476
Cell. 2015 May 7;161(4):919-32
pubmed: 25957690
FEBS Lett. 2003 Feb 27;537(1-3):139-45
pubmed: 12606046
EMBO J. 2015 Mar 12;34(6):778-97
pubmed: 25672362
Mol Biol Cell. 2012 Aug;23(16):3041-56
pubmed: 22718905
Annu Rev Microbiol. 2019 Sep 8;73:89-110
pubmed: 31091419
PLoS Genet. 2019 Oct 25;15(10):e1008479
pubmed: 31652260
Cell Stress Chaperones. 2020 Jul;25(4):573-580
pubmed: 32270443
PLoS One. 2017 Dec 11;12(12):e0189445
pubmed: 29228038
Methods. 2003 Aug;30(4):313-21
pubmed: 12828945
Curr Biol. 2002 Sep 17;12(18):1566-73
pubmed: 12372248
Mol Biol Cell. 1992 Feb;3(2):221-33
pubmed: 1550963
Nat Commun. 2016 Nov 30;7:13673
pubmed: 27901028
Nat Commun. 2019 Oct 24;10(1):4851
pubmed: 31649258
Nat Rev Mol Cell Biol. 2010 Nov;11(11):777-88
pubmed: 20944667
Yeast. 2004 Aug;21(11):947-62
pubmed: 15334558
J Cell Biol. 2010 Sep 6;190(5):719-29
pubmed: 20819932
FASEB J. 2004 Mar;18(3):598-9
pubmed: 14734639
Aging Cell. 2017 Dec;16(6):1414-1424
pubmed: 29024389
Cell Rep. 2016 Jul 19;16(3):826-38
pubmed: 27373154
Mol Microbiol. 2003 Oct;50(2):585-95
pubmed: 14617181
Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20407-12
pubmed: 23184973
Genome Res. 2005 May;15(5):603-15
pubmed: 15837805
PLoS Genet. 2013 Mar;9(3):e1003351
pubmed: 23555277
J Cell Biol. 2011 Nov 14;195(4):617-29
pubmed: 22065637
J Biol Chem. 2017 Jan 13;292(2):672-684
pubmed: 27909051
J Biol Chem. 2020 Jan 3;295(1):158-169
pubmed: 31767683
Mol Cell. 2008 Feb 1;29(2):207-16
pubmed: 18243115
Trends Biochem Sci. 2012 Mar;37(3):106-17
pubmed: 22177323
Aging Cell. 2013 Dec;12(6):1073-81
pubmed: 23879233
Mol Biol Cell. 2004 Feb;15(2):657-64
pubmed: 14668486
Nat Methods. 2011 Sep 04;8(10):879-84
pubmed: 21892152
PLoS Biol. 2014 Jun 17;12(6):e1001886
pubmed: 24936793
Cell. 2013 Jul 3;154(1):134-45
pubmed: 23791384
J Mol Biol. 2015 Apr 10;427(7):1537-48
pubmed: 25681016
J Mol Biol. 2015 Apr 10;427(7):1564-74
pubmed: 25681695
Aging Cell. 2003 Apr;2(2):131-9
pubmed: 12882326
BMC Genomics. 2016 Aug 05;17:559
pubmed: 27496166
EMBO J. 2013 May 15;32(10):1451-68
pubmed: 23604074
Cell. 2014 Jul 31;158(3):549-63
pubmed: 25042851
J Biol Chem. 2020 Mar 6;295(10):3064-3079
pubmed: 32001616
J Cell Biol. 2018 Apr 2;217(4):1269-1285
pubmed: 29362223
Elife. 2015 Nov 06;4:
pubmed: 26544680
Autophagy. 2020 May;16(5):878-899
pubmed: 31354022
EMBO Rep. 2017 Mar;18(3):377-391
pubmed: 28193623
Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):E3780-9
pubmed: 24043785
Cell Rep. 2017 Aug 29;20(9):2100-2115
pubmed: 28854361
J Mol Biol. 2009 Feb 13;386(1):178-89
pubmed: 19101567
Science. 1997 Aug 15;277(5328):942-6
pubmed: 9252323
Aging Cell. 2016 Apr;15(2):217-26
pubmed: 26705243
Cold Spring Harb Perspect Biol. 2011 Aug 01;3(8):a004374
pubmed: 21746797
Curr Genet. 2016 Nov;62(4):711-724
pubmed: 27032776
Plant Cell. 2005 Feb;17(2):559-71
pubmed: 15659638
Nat Cell Biol. 2013 Oct;15(10):1231-43
pubmed: 24036477
Mol Cell. 2015 Jun 18;58(6):1067-78
pubmed: 26009280
Biochimie. 1996;78(2):117-22
pubmed: 8818220
J Vis Exp. 2015 Jan 08;(95):52321
pubmed: 25591151
Science. 2003 May 16;300(5622):1142-5
pubmed: 12750521
J Gerontol A Biol Sci Med Sci. 2001 Jul;56(7):B281-7
pubmed: 11445592
PLoS Genet. 2014 Jul 31;10(7):e1004539
pubmed: 25079602
PLoS One. 2015 Apr 24;10(4):e0124633
pubmed: 25909470
Mol Cell. 2020 Apr 16;78(2):329-345.e9
pubmed: 32268122
Cell Mol Life Sci. 2014 Sep;71(17):3339-61
pubmed: 24458371
Mech Ageing Dev. 2002 Nov;123(12):1531-41
pubmed: 12470891
Genetics. 2018 Nov;210(3):781-787
pubmed: 30213854