There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments.

KCa3.1 NaV1.5 NaV1.7 automated patch clamp fluoride hERG ion channels physiological solutions

Journal

Frontiers in molecular neuroscience
ISSN: 1662-5099
Titre abrégé: Front Mol Neurosci
Pays: Switzerland
ID NLM: 101477914

Informations de publication

Date de publication:
2022
Historique:
received: 30 06 2022
accepted: 26 07 2022
entrez: 8 9 2022
pubmed: 9 9 2022
medline: 9 9 2022
Statut: epublish

Résumé

Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings. In manual patch clamp, fluoride has been used to record voltage-gated Na (Na

Identifiants

pubmed: 36072300
doi: 10.3389/fnmol.2022.982316
pmc: PMC9443850
doi:

Types de publication

Journal Article

Langues

eng

Pagination

982316

Informations de copyright

Copyright © 2022 Rapedius, Obergrussberger, Humphries, Scholz, Rinke-Weiss, Goetze, Brinkwirth, Rotordam, Strassmaier, Randolph, Friis, Liutkute, Seibertz, Voigt and Fertig.

Déclaration de conflit d'intérêts

MR, AO, SS, IR-W, TG, NB, MGR, TS, AR, SF, and NF were employed by Nanion Technologies, the manufacturers of the SyncroPatch 384 used to compile this manuscript. NF was a shareholder in Nanion Technologies. EH was employed by BioPharmaceuticals R&D, AstraZeneca. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Markus Rapedius (M)

Nanion Technologies GmbH, Munich, Germany.

Alison Obergrussberger (A)

Nanion Technologies GmbH, Munich, Germany.

Edward S A Humphries (ESA)

Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, Cambridge, United Kingdom.

Stephanie Scholz (S)

Nanion Technologies GmbH, Munich, Germany.

Ilka Rinke-Weiss (I)

Nanion Technologies GmbH, Munich, Germany.

Tom A Goetze (TA)

Nanion Technologies GmbH, Munich, Germany.

Nina Brinkwirth (N)

Nanion Technologies GmbH, Munich, Germany.

Maria Giustina Rotordam (MG)

Nanion Technologies GmbH, Munich, Germany.

Tim Strassmaier (T)

Nanion Technologies Inc., Livingston, NJ, United States.

Aaron Randolph (A)

Nanion Technologies Inc., Livingston, NJ, United States.

Søren Friis (S)

Nanion Technologies GmbH, Munich, Germany.

Aiste Liutkute (A)

Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Georg-August University, Göttingen, Germany.
German Center for Cardiovascular Research, Partner Site Göttingen, Göttingen, Germany.
Cluster of Excellence "Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells", University of Göttingen, Göttingen, Germany.

Fitzwilliam Seibertz (F)

Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Georg-August University, Göttingen, Germany.
German Center for Cardiovascular Research, Partner Site Göttingen, Göttingen, Germany.
Cluster of Excellence "Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells", University of Göttingen, Göttingen, Germany.

Niels Voigt (N)

Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Georg-August University, Göttingen, Germany.
German Center for Cardiovascular Research, Partner Site Göttingen, Göttingen, Germany.
Cluster of Excellence "Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells", University of Göttingen, Göttingen, Germany.

Niels Fertig (N)

Nanion Technologies GmbH, Munich, Germany.

Classifications MeSH