New data analysis for BioSAXS at the ESRF.
BioSAXS
automation
biological small-angle X-ray scattering
high brilliance
high-throughput SAXS
online data analysis
online purification
proteins
size-exclusion chromatography
solution scattering
structural biology
Journal
Journal of synchrotron radiation
ISSN: 1600-5775
Titre abrégé: J Synchrotron Radiat
Pays: United States
ID NLM: 9888878
Informations de publication
Date de publication:
01 Sep 2022
01 Sep 2022
Historique:
received:
06
05
2022
accepted:
13
07
2022
entrez:
8
9
2022
pubmed:
9
9
2022
medline:
11
9
2022
Statut:
ppublish
Résumé
The second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a new wiggler source and a larger detector. All analysis software has been rewritten to cope with the increased data flux and continues to provide beamline users with reduced and pre-processed data in real time. This article describes FreeSAS, an open-source collection of various small-angle scattering analysis algorithms needed to reduce and analyze BioSAXS data, and Dahu, the tool used to interface data analysis with beamline control. It further presents the data-processing pipelines for the different data acquisitions modes of the beamline, using either a sample changer for individual homogeneous samples or an inline size-exclusion chromatography setup.
Identifiants
pubmed: 36073892
pii: S1600577522007238
doi: 10.1107/S1600577522007238
pmc: PMC9455220
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1318-1328Informations de copyright
open access.
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