Structural and functional characterization of NEMO cleavage by SARS-CoV-2 3CLpro.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
08 09 2022
Historique:
received: 18 11 2021
accepted: 23 08 2022
entrez: 8 9 2022
pubmed: 9 9 2022
medline: 14 9 2022
Statut: epublish

Résumé

In addition to its essential role in viral polyprotein processing, the SARS-CoV-2 3C-like protease (3CLpro) can cleave human immune signaling proteins, like NF-κB Essential Modulator (NEMO) and deregulate the host immune response. Here, in vitro assays show that SARS-CoV-2 3CLpro cleaves NEMO with fine-tuned efficiency. Analysis of the 2.50 Å resolution crystal structure of 3CLpro C145S bound to NEMO

Identifiants

pubmed: 36075915
doi: 10.1038/s41467-022-32922-9
pii: 10.1038/s41467-022-32922-9
pmc: PMC9453703
doi:

Substances chimiques

Antiviral Agents 0
Proteins 0
Peptide Hydrolases EC 3.4.-
Cysteine Endopeptidases EC 3.4.22.-

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

5285

Subventions

Organisme : NIGMS NIH HHS
ID : P30 GM133894
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG053303
Pays : United States

Commentaires et corrections

Type : UpdateOf

Informations de copyright

© 2022. The Author(s).

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Auteurs

Mikhail A Hameedi (MA)

SLAC National Accelerator Laboratory, Stanford Synchrotron Radiation Lightsource, Structural Molecular Biology, Menlo Park, CA, 94025, USA.
SLAC National Accelerator Laboratory, Stanford Synchrotron Radiation Lightsource, Biosciences, Menlo Park, CA, 94025, USA.
Department of Structural Biology, Stanford University, Stanford, CA, 94305, USA.
National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA.

Erica T Prates (E)

National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Michael R Garvin (MR)

National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Irimpan I Mathews (II)

SLAC National Accelerator Laboratory, Stanford Synchrotron Radiation Lightsource, Structural Molecular Biology, Menlo Park, CA, 94025, USA.

B Kirtley Amos (BK)

Department of Horticulture, University of Kentucky, Lexington, KY, USA.

Omar Demerdash (O)

National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Mark Bechthold (M)

Department of Structural Biology, Stanford University, Stanford, CA, 94305, USA.

Mamta Iyer (M)

Chapman University School of Pharmacy, Irvine, CA, 92618, USA.

Simin Rahighi (S)

Chapman University School of Pharmacy, Irvine, CA, 92618, USA.

Daniel W Kneller (DW)

National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA.
Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Andrey Kovalevsky (A)

National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA.
Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Stephan Irle (S)

Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.

Van-Quan Vuong (VQ)

The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee Knoxville, Knoxville, TN, USA.

Julie C Mitchell (JC)

National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Audrey Labbe (A)

National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Stephanie Galanie (S)

National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
Department of Process Research and Development, Merck & Co., Inc., Rahway, NJ, 07065, USA.

Soichi Wakatsuki (S)

SLAC National Accelerator Laboratory, Stanford Synchrotron Radiation Lightsource, Structural Molecular Biology, Menlo Park, CA, 94025, USA. soichi.wakatsuki@stanford.edu.
SLAC National Accelerator Laboratory, Stanford Synchrotron Radiation Lightsource, Biosciences, Menlo Park, CA, 94025, USA. soichi.wakatsuki@stanford.edu.
Department of Structural Biology, Stanford University, Stanford, CA, 94305, USA. soichi.wakatsuki@stanford.edu.
National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA. soichi.wakatsuki@stanford.edu.

Daniel Jacobson (D)

National Virtual Biotechnology Laboratory, US Department of Energy, Washington, DC, USA. jacobsonda@ornl.gov.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. jacobsonda@ornl.gov.

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