Liposome-Encapsulated Bioactive Guttiferone E Exhibits Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated MH-S Macrophages and Cytotoxicity against Human Cancer Cells.
Journal
Mediators of inflammation
ISSN: 1466-1861
Titre abrégé: Mediators Inflamm
Pays: United States
ID NLM: 9209001
Informations de publication
Date de publication:
2022
2022
Historique:
received:
05
07
2022
revised:
15
08
2022
accepted:
17
08
2022
entrez:
9
9
2022
pubmed:
10
9
2022
medline:
14
9
2022
Statut:
epublish
Résumé
Guttiferone E is a naturally occurring polyisoprenylated benzophenone exhibiting a wide range of remarkable biological activities. But its therapeutic application is still limited due to its poor water solubility. This study is aimed at preparing guttiferone E-loaded liposomes and assessing their Liposomes containing guttiferone E were prepared by the thin film hydration method, and the physicochemical characteristics were determined using dynamic light scattering, laser Doppler velocimetry, and atomic force microscopy. The cytotoxicity was assessed by the MTT assay. The fluorometric cyclooxygenase (COX) activity assay kit was used to assess the COX activity while the nitric oxide production was evaluated by the Griess reagent method. The liposomes with a mean size of 183.33 ± 17.28 nm were obtained with an entrapment efficiency of 63.86%. Guttiferone E-loaded liposomes successfully decreased the viability of cancer cells. The overall IC This study indicates that liposomes can be an alternative to overcome the water insolubility issue of the bioactive guttiferone E.
Sections du résumé
Background
UNASSIGNED
Guttiferone E is a naturally occurring polyisoprenylated benzophenone exhibiting a wide range of remarkable biological activities. But its therapeutic application is still limited due to its poor water solubility. This study is aimed at preparing guttiferone E-loaded liposomes and assessing their
Methods
UNASSIGNED
Liposomes containing guttiferone E were prepared by the thin film hydration method, and the physicochemical characteristics were determined using dynamic light scattering, laser Doppler velocimetry, and atomic force microscopy. The cytotoxicity was assessed by the MTT assay. The fluorometric cyclooxygenase (COX) activity assay kit was used to assess the COX activity while the nitric oxide production was evaluated by the Griess reagent method.
Results
UNASSIGNED
The liposomes with a mean size of 183.33 ± 17.28 nm were obtained with an entrapment efficiency of 63.86%. Guttiferone E-loaded liposomes successfully decreased the viability of cancer cells. The overall IC
Conclusion
UNASSIGNED
This study indicates that liposomes can be an alternative to overcome the water insolubility issue of the bioactive guttiferone E.
Identifiants
pubmed: 36081652
doi: 10.1155/2022/8886087
pmc: PMC9448579
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Benzophenones
0
Lipopolysaccharides
0
Liposomes
0
Water
059QF0KO0R
Nitric Oxide
31C4KY9ESH
guttiferone E
74F950KV9S
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8886087Informations de copyright
Copyright © 2022 Jean Paul Dzoyem et al.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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