The reference genome of the Vernal Pool Tadpole Shrimp, Lepidurus packardi.

CCGP California Conservation Genomics Project branchiopod notostraca triops

Journal

The Journal of heredity
ISSN: 1465-7333
Titre abrégé: J Hered
Pays: United States
ID NLM: 0375373

Informations de publication

Date de publication:
30 11 2022
Historique:
received: 16 08 2022
accepted: 07 09 2022
pubmed: 10 9 2022
medline: 2 12 2022
entrez: 9 9 2022
Statut: ppublish

Résumé

In this paper, we report on the scaffold-level assembled genome for the federally endangered, California endemic crustacean Lepidurus packardi (the Vernal Pool Tadpole Shrimp). L. packardi is a key food source for other conserved California species including the California Tiger Salamander Ambystoma californiense. It faces significant habitat loss and fragmentation as vernal pools are threatened by urbanization, agricultural conversion, and climate change. This resource represents the first scaffold-level genome of any Lepidurus species. The assembled genome spans 108.6 Mbps, with 6 chromosome-length scaffolds comprising 71% of total genomic length and 444 total contigs. The BUSCO score for this genome is 97.3%, suggesting a high level of completeness. We produced a predicted gene set for this species trained on the Daphnia magna set of genes and predicted 17,650 genes. These tools can aid researchers in understanding the evolution and adaptive potential of alternative reproductive modes within this species.

Identifiants

pubmed: 36082700
pii: 6694764
doi: 10.1093/jhered/esac051
pmc: PMC9709966
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

706-711

Subventions

Organisme : NIH HHS
ID : S10 OD010786
Pays : United States

Informations de copyright

© The American Genetic Association. 2022.

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Auteurs

Shannon Rose Kieran Blair (SR)

Genomic Variation Laboratory, Department of Animal Science, University of California, Davis, Davis, CA, United States.

Joshua Hull (J)

U.S. Fish and Wildlife Service, Sacramento Fish and Wildlife Office, Sacramento, CA, United States.

Merly Escalona (M)

Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, United States.

Amanda Finger (A)

Genomic Variation Laboratory, Department of Animal Science, University of California, Davis, Davis, CA, United States.

Shannon E K Joslin (SEK)

U.S. National Park Service, Yosemite National Park, El Portal, CA, United States.

Ruta Sahasrabudhe (R)

DNA Technologies and Expression Analysis Cores, UC Davis Genome Center, University of California, Davis, Davis, CA, United States.

Mohan P A Marimuthu (MPA)

DNA Technologies and Expression Analysis Cores, UC Davis Genome Center, University of California, Davis, Davis, CA, United States.

Oanh Nguyen (O)

DNA Technologies and Expression Analysis Cores, UC Davis Genome Center, University of California, Davis, Davis, CA, United States.

Noravit Chumchim (N)

DNA Technologies and Expression Analysis Cores, UC Davis Genome Center, University of California, Davis, Davis, CA, United States.

Emily Reister Morris (ER)

Phase Genomics, Seattle, WA, United States.

Samantha Velazquez (S)

Phase Genomics, Seattle, WA, United States.

Andrea Schreier (A)

Genomic Variation Laboratory, Department of Animal Science, University of California, Davis, Davis, CA, United States.

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Classifications MeSH