Analysis of cryoproteins with a focus on cryofibrinogen: a study on 103 patients.
cryofibrinogen
cryoglobulins
cryoproteins
Journal
Clinical chemistry and laboratory medicine
ISSN: 1437-4331
Titre abrégé: Clin Chem Lab Med
Pays: Germany
ID NLM: 9806306
Informations de publication
Date de publication:
26 10 2022
26 10 2022
Historique:
received:
29
04
2022
accepted:
31
08
2022
pubmed:
10
9
2022
medline:
5
10
2022
entrez:
9
9
2022
Statut:
epublish
Résumé
Cryofibrinogen (CF) is an abnormal protein in plasma that precipitates at 4 °C and dissolves at 37 °C. Whilst serum cryoglobulins (CGs) analysis is common practice, CF investigation is rarely performed. This study aims to describe the testing methodology developed at our laboratory, potential pitfalls for all analytical phases, the distribution among hospital wards and clinical conditions underlying test requests and clinical conditions in which to order CF analysis is useful. Retrospective analysis of laboratory samples received between January 2019 and June 2021 with CF testing requests. A complete protocol for CF pre-analytical, analytical and post-analytical phases are supplied. Most test requests were received from the rheumatology department for systemic sclerosis or liver transplant screening. Among the 103 in-patients included, CF+ was confirmed in 68 patients (66%). Of observed CF+ patients (n=68) most cases were CGs- (n=44, 67%). Isolated CF was found in 43% of the cases. Among CF- patients (n=35; 34%) only 2 patients had positive CGs (CGs+). Among rheumatology patients (n=66), isolated CF+ was observed in 45% (n=30/66), whilst among patients with systemic sclerosis with CF+ (n=19), isolated CF+ was detected in 79% (n=15/19). Described analytical procedures may be used for the creation of harmonized recommendations and indications for CF analysis. Isolated CF positivity among hospitalized patients, predominantly rheumatology and systemic sclerosis patients, appears higher than rates previously reported in literature. We propose CF test recommendations should be included in investigation protocols for diseases where cryofibrinogenemia may occur.
Identifiants
pubmed: 36082756
pii: cclm-2022-0423
doi: 10.1515/cclm-2022-0423
doi:
Substances chimiques
Cryoglobulins
0
Fibrinogens, Abnormal
0
cryofibrinogen
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1796-1803Informations de copyright
© 2022 Walter de Gruyter GmbH, Berlin/Boston.
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