Cloning and characterization of the major AP endonuclease from Staphylococcus aureus.

Cloning, Molecular DNA / metabolism DNA Damage DNA Repair DNA-(Apurinic or Apyrimidinic Site) Lyase / metabolism Deoxyribonuclease IV (Phage T4-Induced) / chemistry Endonucleases / metabolism Escherichia coli / metabolism Hydrogen Peroxide Nucleotides Staphylococcus aureus / genetics
AP endonuclease Abasic sites Base excision repair DNA damage DNA repair Endonuclease IV Nucleotide incision repair Oxidative damage Staphylococcus aureus

Journal

DNA repair
ISSN: 1568-7856
Titre abrégé: DNA Repair (Amst)
Pays: Netherlands
ID NLM: 101139138

Informations de publication

Date de publication:
11 2022
Historique:
received: 25 04 2022
revised: 19 07 2022
accepted: 20 08 2022
pubmed: 12 9 2022
medline: 14 10 2022
entrez: 11 9 2022
Statut: ppublish

Résumé

Apurinic/apyrimidinic (AP) endonucleases are key enzymes involved in the repair of abasic sites and DNA strand breaks. Complete genome analysis of Staphylococcus aureus identified a single AP endonuclease, SaNfo, which is a member of the endonuclease IV family exemplified by Escherichia coli Nfo. At present, it remains unknown whether SaNfo possesses DNA repair activities similar to its counterparts from E. coli and other bacteria. Here, we report that the purified SaNfo protein contains efficient AP endonuclease and nucleotide incision repair (NIR) activities. Optimal reaction conditions for SaNfo-catalysed AP endonuclease activity are high ionic strength and Mn

Identifiants

DOI: 10.1016/j.dnarep.2022.103390 PMID: 36088709
pubmed: 36088709
pii: S1568-7864(22)00123-9
doi: 10.1016/j.dnarep.2022.103390
pii:
doi:

Substances chimiques

Nucleotides 0
DNA 9007-49-2
Hydrogen Peroxide BBX060AN9V
Endonucleases EC 3.1.-
Deoxyribonuclease IV (Phage T4-Induced) EC 3.1.21.2
DNA-(Apurinic or Apyrimidinic Site) Lyase EC 4.2.99.18

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

103390

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest The authors declare that there are no conflicts of interest.

Auteurs

Aigerim Turgimbayeva (A)

National Center for Biotechnology, Nur-Sultan 010000, Kazakhstan; L. N. Gumilyov Eurasian National University, Nur-Sultan 010000, Kazakhstan.

Ulan Zein (U)

National Center for Biotechnology, Nur-Sultan 010000, Kazakhstan.

Dmitry O Zharkov (DO)

SB RAS Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, Russia; Novosibirsk State University, Novosibirsk 630090, Russia.

Yerlan Ramankulov (Y)

National Center for Biotechnology, Nur-Sultan 010000, Kazakhstan; School of Science and Humanities, Nazarbayev University, Qabanbay Batyr Ave 53, Nur-Sultan 010000, Kazakhstan.

Murat Saparbaev (M)

Groupe "Mechanisms of DNA Repair and Carcinogenesis", CNRS UMR9019, Université Paris-Saclay, Gustave Roussy Cancer Campus, F-94805 Villejuif Cedex, France.

Sailau Abeldenov (S)

National Center for Biotechnology, Nur-Sultan 010000, Kazakhstan. Electronic address: abeldenov@gmail.com.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › Cloning and characterization of the major AP...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Cloning and characterization of the major AP...
questionsmedicales.fr › Phénomènes génétiques › Altération de l'ADN › Cloning and characterization of the major AP...
questionsmedicales.fr › Phénomènes génétiques › Réparation de l'ADN › Cloning and characterization of the major AP...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-oxygen lyases › DNA-(apurinic or apyrimidinic site) lyase › Cloning and characterization of the major AP...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Désoxyribonucléases › Deoxyribonuclease IV (phage T4-induced) › Cloning and characterization of the major AP...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Endonucleases › Cloning and characterization of the major AP...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Cloning and characterization of the major AP...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › Cloning and characterization of the major AP...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Cloning and characterization of the major AP...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Cocci à Gram positif › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Cloning and characterization of the major AP...