A combined strategy for the overproduction of complex ergot alkaloid agroclavine.
Agroclavine
Cell-free systems
Ergot alkaloids
Microbial cell factories
Overproduction
Journal
Synthetic and systems biotechnology
ISSN: 2405-805X
Titre abrégé: Synth Syst Biotechnol
Pays: China
ID NLM: 101694371
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
25
06
2022
revised:
10
08
2022
accepted:
16
08
2022
entrez:
12
9
2022
pubmed:
13
9
2022
medline:
13
9
2022
Statut:
epublish
Résumé
Microbial cell factories (MCFs) and cell-free systems (CFSs) are generally considered as two unrelated approaches for the biosynthesis of biomolecules. In the current study, two systems were combined together for the overproduction of agroclavine (AC), a structurally complex ergot alkaloid. The whole biosynthetic pathway for AC was split into the early pathway and the late pathway at the point of the FAD-linked oxidoreductase EasE, which was reconstituted in an MCF (
Identifiants
pubmed: 36092273
doi: 10.1016/j.synbio.2022.08.003
pii: S2405-805X(22)00084-9
pmc: PMC9428804
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1126-1132Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled.
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