Live-cell metabolic analysis of oligodendroglia isolated from postnatal mouse brain and spinal cord.
Cell isolation
Cell-based assays
Metabolism
Neuroscience
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
16 09 2022
16 09 2022
Historique:
entrez:
12
9
2022
pubmed:
13
9
2022
medline:
14
9
2022
Statut:
epublish
Résumé
This protocol describes isolation and live-cell metabolic analysis of O4+ oligodendroglia from brain and spinal cord of postnatal mice. We have optimized existing protocols for O4+ isolation from neonatal brain and expanded the protocol to include isolation of highly viable oligodendroglia from spinal cords of postnatal mice up to 18 days of age. Isolated oligodendroglia can be used in multiple downstream analyses, and here we describe an optimized real-time metabolic assay using Agilent Seahorse Analyzer to measure mitochondrial respiration. For complete details on the use and execution of this protocol, please refer to Khandker et al. (2022).
Identifiants
pubmed: 36092821
doi: 10.1016/j.xpro.2022.101655
pii: S2666-1667(22)00535-4
pmc: PMC9449657
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101655Subventions
Organisme : NINDS NIH HHS
ID : R37 NS082203
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS082203
Pays : United States
Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests. L.K. is currently a Senior Scientist at Palatin Technologies, Cranbury, NJ 08512.
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