Fluorochromized tyramide-glucose oxidase as a multiplex fluorescent tyramide signal amplification system for histochemical analysis.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
12 09 2022
12 09 2022
Historique:
received:
11
05
2022
accepted:
24
08
2022
entrez:
13
9
2022
pubmed:
14
9
2022
medline:
15
9
2022
Statut:
epublish
Résumé
Tyramide signal amplification (TSA) is a highly sensitive method for histochemical analysis. Previously, we reported a TSA system, biotinyl tyramine-glucose oxidase (BT-GO), for bright-filed imaging. Here, we develop fluorochromized tyramide-glucose oxidase (FT-GO) as a multiplex fluorescent TSA system. FT-GO involves peroxidase-catalyzed deposition of fluorochromized tyramide (FT) with hydrogen peroxide produced by enzymatic reaction between glucose and glucose oxidase. We showed that FT-GO enhanced immunofluorescence signals while maintaining low background signals. Compared with indirect immunofluorescence detections, FT-GO demonstrated a more widespread distribution of monoaminergic projection systems in mouse and marmoset brains. For multiplex labeling with FT-GO, we quenched antibody-conjugated peroxidase using sodium azide. We applied FT-GO to multiplex fluorescent in situ hybridization, and succeeded in labeling neocortical interneuron subtypes by coupling with immunofluorescence. FT-GO immunofluorescence further increased the detectability of an adeno-associated virus tracer. Given its simplicity and a staining with a high signal-to-noise ratio, FT-GO would provide a versatile platform for histochemical analysis.
Identifiants
pubmed: 36097273
doi: 10.1038/s41598-022-19085-9
pii: 10.1038/s41598-022-19085-9
pmc: PMC9468149
doi:
Substances chimiques
Coloring Agents
0
Glucose Oxidase
EC 1.1.3.4
Peroxidases
EC 1.11.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14807Informations de copyright
© 2022. The Author(s).
Références
J Neurosci. 2012 Jun 6;32(23):7970-85
pubmed: 22674272
J Histochem Cytochem. 1995 Apr;43(4):347-52
pubmed: 7897179
J Neurosci. 2014 Jan 22;34(4):1258-70
pubmed: 24453317
STAR Protoc. 2020 Dec 18;2(1):100230
pubmed: 33364620
Virology. 2008 Mar 1;372(1):24-34
pubmed: 18035387
Histochem Cell Biol. 1997 Oct-Nov;108(4-5):325-33
pubmed: 9387925
J Immunol Methods. 1991 Mar 1;137(1):103-12
pubmed: 1849153
Development. 1992 Sep;116(1):201-11
pubmed: 1483388
Elife. 2018 Nov 20;7:
pubmed: 30454553
iScience. 2020 Aug 6;:101409
pubmed: 32877648
Cereb Cortex. 2011 Nov;21(11):2639-49
pubmed: 21467210
Curr Protoc Neurosci. 2012 Apr;Chapter 1:Unit 1.20.1-18
pubmed: 22470147
J Immunol Methods. 1992 Jun 24;150(1-2):145-9
pubmed: 1613251
Nature. 2019 Sep;573(7772):61-68
pubmed: 31435019
J Histochem Cytochem. 1992 Oct;40(10):1457-63
pubmed: 1527370
Neuron. 2016 Apr 20;90(2):219-33
pubmed: 27100195
Cereb Cortex. 2009 Sep;19(9):2065-77
pubmed: 19174446
Cytometry. 1996 Jan 1;23(1):48-53
pubmed: 14650440
Hum Mol Genet. 1995 Apr;4(4):529-34
pubmed: 7633400
J Comp Neurol. 2003 Dec 1;467(1):60-79
pubmed: 14574680
Elife. 2021 Jul 14;10:
pubmed: 34259630
BMC Dev Biol. 2013 Mar 12;13:8
pubmed: 23497040
J Cell Biol. 2018 Nov 5;217(11):4025-4048
pubmed: 30154186
J Histochem Cytochem. 1994 Dec;42(12):1635-42
pubmed: 7983364
J Histochem Cytochem. 2007 Jun;55(6):545-54
pubmed: 17242468
Methods. 2014 Nov;70(1):46-58
pubmed: 25242720
J Comp Neurol. 2014 Jun 15;522(9):1989-2012
pubmed: 24639291
Gene Ther. 2021 Feb;28(1-2):56-74
pubmed: 32576975
Neuron. 2007 Oct 4;56(1):33-42
pubmed: 17920013
Hum Gene Ther. 2012 Jul;23(7):742-53
pubmed: 22471423
Nat Biotechnol. 2019 Sep;37(9):1080-1090
pubmed: 31427819
J Immunol Methods. 1989 Dec 20;125(1-2):279-85
pubmed: 2558138
Mol Ther. 2006 Mar;13(3):517-27
pubmed: 16325474
Cereb Cortex. 2005 Jan;15(1):96-108
pubmed: 15217901
Cell. 2019 Sep 19;179(1):268-281.e13
pubmed: 31495573
J Neurosci. 2009 Apr 1;29(13):4089-95
pubmed: 19339604
J Neurosci. 2009 Jan 14;29(2):444-53
pubmed: 19144844
Hum Gene Ther. 1999 Sep 20;10(14):2295-305
pubmed: 10515449
Neuron. 2016 Nov 2;92(3):582-590
pubmed: 27809998
Neurosci Res. 2015 Apr;93:144-57
pubmed: 25240284
J Histochem Cytochem. 1996 Apr;44(4):389-92
pubmed: 8601698
Nature. 2021 Oct;598(7879):86-102
pubmed: 34616075
J Comp Neurol. 2010 Mar 1;518(5):668-86
pubmed: 20034056
J Neurosci. 2001 Sep 15;21(18):7153-60
pubmed: 11549726
Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82
pubmed: 12060735
Nature. 2014 Apr 10;508(7495):207-14
pubmed: 24695228
Front Neuroanat. 2020 Jul 21;14:32
pubmed: 32792913
iScience. 2021 Dec 27;25(1):103601
pubmed: 35106459
Science. 2004 Aug 6;305(5685):846
pubmed: 15297669
Nature. 2018 Nov;563(7729):72-78
pubmed: 30382198
PLoS One. 2017 Jan 6;12(1):e0169611
pubmed: 28060929
Brain Res Bull. 2020 Sep;162:94-106
pubmed: 32562720
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Pharmacol Toxicol. 2002 Dec;91(6):382-6
pubmed: 12688383
Acta Histochem. 1997 Nov;99(4):411-29
pubmed: 9429601
J Histochem Cytochem. 1997 Nov;45(11):1449-54
pubmed: 9358846
eNeuro. 2015 Mar-Apr;2(2):
pubmed: 26023683
J Comp Neurol. 2006 Nov 10;499(2):258-73
pubmed: 16977617
Elife. 2019 Oct 24;8:
pubmed: 31647409