Co-culture of primary human T cells with leukemia cells to measure regulatory T cell expansion.
Cancer
Cell Biology
Cell culture
Cell isolation
Cell-based Assays
Flow Cytometry/Mass Cytometry
Health Sciences
Immunology
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
16 09 2022
16 09 2022
Historique:
received:
28
06
2022
revised:
15
07
2022
accepted:
01
08
2022
pubmed:
14
9
2022
medline:
23
9
2022
entrez:
13
9
2022
Statut:
ppublish
Résumé
The expansion of regulatory T cells (Tregs) is known to be mediated by cytokines including IL-10 and TGFβ but has additionally been shown to depend on the interaction of the immune receptors ICOSLG and ICOS. Here, we describe a co-culture system which enables quantification of the ability of leukemia cells to induce Treg expansion through secreted cytokines and direct receptor interactions. The protocol is applicable for MHC-matched and -unmatched experiments and allows assessment of Treg expansion without using a mouse model. For complete details on the use and execution of this protocol, please refer to Külp et al. (2022).
Identifiants
pubmed: 36097388
pii: S2666-1667(22)00541-X
doi: 10.1016/j.xpro.2022.101661
pmc: PMC9471457
pii:
doi:
Substances chimiques
Cytokines
0
Transforming Growth Factor beta
0
Interleukin-10
130068-27-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101661Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare that they have no competing interests.