A DNase Type VI Secretion System Effector Requires Its MIX Domain for Secretion.
DNase
T6SS
Vibrio
competition
effector
secretion systems
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
26 10 2022
26 10 2022
Historique:
pubmed:
14
9
2022
medline:
29
10
2022
entrez:
13
9
2022
Statut:
ppublish
Résumé
Gram-negative bacteria often employ the type VI secretion system (T6SS) to deliver diverse cocktails of antibacterial effectors into rival bacteria. In many cases, even when the identity of the delivered effectors is known, their toxic activity and mechanism of secretion are not. Here, we investigate VPA1263, a Vibrio parahaemolyticus T6SS effector that belongs to a widespread class of polymorphic effectors containing a MIX domain. We reveal a C-terminal DNase toxin domain belonging to the HNH nuclease superfamily, and we show that it mediates the antibacterial toxicity of this effector during bacterial competition. Furthermore, we demonstrate that the VPA1263 MIX domain is necessary for T6SS-mediated secretion and intoxication of recipient bacteria. These results are the first indication of a functional role for MIX domains in T6SS secretion.
Identifiants
pubmed: 36098406
doi: 10.1128/spectrum.02465-22
pmc: PMC9602870
doi:
Substances chimiques
Type VI Secretion Systems
0
Deoxyribonucleases
EC 3.1.-
Bacterial Proteins
0
Anti-Bacterial Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0246522Références
mBio. 2015 May 05;6(3):e00285-15
pubmed: 25944858
Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):7032-7
pubmed: 23572593
Annu Rev Microbiol. 2019 Sep 8;73:621-638
pubmed: 31226022
Genome Res. 2004 Jun;14(6):1188-90
pubmed: 15173120
Cell Host Microbe. 2010 Jan 21;7(1):25-37
pubmed: 20114026
EMBO Rep. 2022 Jan 5;23(1):e53981
pubmed: 34752000
Lancet. 2003 Mar 1;361(9359):743-9
pubmed: 12620739
EMBO Rep. 2021 Nov 4;22(11):e53681
pubmed: 34494702
Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3931-40
pubmed: 27313214
Mol Cell. 2013 Sep 12;51(5):584-93
pubmed: 23954347
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Elife. 2017 Jul 11;6:
pubmed: 28696203
Annu Rev Microbiol. 2020 Sep 8;74:497-520
pubmed: 32680451
Biochimie. 2002 May-Jun;84(5-6):499-510
pubmed: 12423794
Mol Microbiol. 2008 May;68(4):838-47
pubmed: 18430080
J Mol Biol. 2018 Jul 20;430(15):2237-2243
pubmed: 29258817
PLoS One. 2014 Feb 26;9(2):e89313
pubmed: 24586681
PLoS One. 2013 Apr 16;8(4):e61086
pubmed: 23613791
Mol Microbiol. 1996 Feb;19(3):625-37
pubmed: 8830252
Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):6022-7
pubmed: 24711378
Nat Microbiol. 2018 May;3(5):632-640
pubmed: 29632369
Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6
pubmed: 24927539
Nat Microbiol. 2018 Oct;3(10):1142-1152
pubmed: 30177742
Cell Host Microbe. 2012 May 17;11(5):538-49
pubmed: 22607806
Biol Direct. 2012 Jun 25;7:18
pubmed: 22731697
J Bacteriol. 1993 May;175(10):2907-16
pubmed: 8491711
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6791-6
pubmed: 10829063
Microbiology (Reading). 2014 Sep;160(Pt 9):1867-1873
pubmed: 24987102
PLoS Pathog. 2016 Jun 28;12(6):e1005735
pubmed: 27352036
Anal Biochem. 1976 Jan;70(1):241-50
pubmed: 1259145
mBio. 2022 Apr 26;13(2):e0339621
pubmed: 35343790
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):9427-9432
pubmed: 28808000
PLoS Pathog. 2012;8(3):e1002593
pubmed: 22438811
BMC Genomics. 2006 May 03;7:104
pubmed: 16672049
EMBO Rep. 2017 Nov;18(11):1978-1990
pubmed: 28912123
Mol Microbiol. 2021 Mar;115(3):383-394
pubmed: 33217073
J Bacteriol. 2004 Jul;186(13):4307-14
pubmed: 15205433
Future Microbiol. 2019 Nov;14:1451-1463
pubmed: 31718312
Nucleic Acids Res. 2018 Jul 2;46(W1):W200-W204
pubmed: 29905871
J Gen Microbiol. 1991 Jan;137(1):91-100
pubmed: 2045785
Mar Drugs. 2018 Nov 04;16(11):
pubmed: 30400344
BMC Genomics. 2009 Mar 12;10:104
pubmed: 19284603
Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1528-33
pubmed: 16432199
Nature. 2012 Feb 26;483(7388):182-6
pubmed: 22367545
Nucleic Acids Res. 2004 Nov 23;32(20):6129-35
pubmed: 15562004
J Biol Chem. 2014 Jun 20;289(25):17872-84
pubmed: 24794869
Nat Commun. 2019 Aug 9;10(1):3595
pubmed: 31399579
Nature. 2010 Nov 18;468(7322):439-42
pubmed: 21085179
EMBO J. 2015 Aug 13;34(16):2198-210
pubmed: 26194724
Nucleic Acids Res. 2007 Jan;35(Database issue):D237-40
pubmed: 17135202
Biochem J. 2016 Sep 15;473(18):2799-812
pubmed: 27402794
Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9106-11
pubmed: 26150500
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Appl Environ Microbiol. 2017 Jun 16;83(13):
pubmed: 28432099
Q Rev Biophys. 2005 Feb;38(1):49-95
pubmed: 16336743
Cell. 2016 Sep 22;167(1):99-110.e12
pubmed: 27616061
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
pubmed: 15034147
PLoS Pathog. 2015 Aug 25;11(8):e1005128
pubmed: 26305100
J Bacteriol. 2015 Jul;197(14):2350-60
pubmed: 25939831
Infect Immun. 2011 Jul;79(7):2941-9
pubmed: 21555399