Interleukin-1 contributes to clonal expansion and progression of bone marrow fibrosis in JAK2V617F-induced myeloproliferative neoplasm.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
13 09 2022
Historique:
received: 09 07 2021
accepted: 24 08 2022
entrez: 13 9 2022
pubmed: 14 9 2022
medline: 16 9 2022
Statut: epublish

Résumé

Chronic inflammation is frequently associated with myeloproliferative neoplasms (MPN), but the role of inflammation in the pathogenesis of MPN remains unclear. Expression of the proinflammatory cytokine interleukin-1 (IL-1) is elevated in patients with MPN as well as in Jak2V617F knock-in mice. Here, we show that genetic deletion of IL-1 receptor 1 (IL-1R1) normalizes peripheral blood counts, reduces splenomegaly and ameliorates bone marrow fibrosis in homozygous Jak2V617F mouse model of myelofibrosis. Deletion of IL-1R1 also significantly reduces Jak2V617F mutant hematopoietic stem/progenitor cells. Exogenous administration of IL-1β enhances myeloid cell expansion and accelerates the development of bone marrow fibrosis in heterozygous Jak2V617F mice. Furthermore, treatment with anti-IL-1R1 antibodies significantly reduces leukocytosis and splenomegaly, and ameliorates bone marrow fibrosis in homozygous Jak2V617F mice. Collectively, these results suggest that IL-1 signaling plays a pathogenic role in MPN disease progression, and targeting of IL-1R1 could be a useful strategy for the treatment of myelofibrosis.

Identifiants

pubmed: 36100596
doi: 10.1038/s41467-022-32928-3
pii: 10.1038/s41467-022-32928-3
pmc: PMC9470702
doi:

Substances chimiques

IL1R1 protein, mouse 0
Interleukin-1 0
Receptors, Interleukin-1 Type I 0
Jak2 protein, mouse EC 2.7.10.2
Janus Kinase 2 EC 2.7.10.2

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

5347

Subventions

Organisme : NCI NIH HHS
ID : P30 CA044579
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL095685
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL149893
Pays : United States

Informations de copyright

© 2022. The Author(s).

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Auteurs

Mohammed Ferdous-Ur Rahman (MF)

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.

Yue Yang (Y)

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.

Bao T Le (BT)

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.

Avik Dutta (A)

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.

Julia Posyniak (J)

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.

Patrick Faughnan (P)

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.

Mohammad A Sayem (MA)

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.

Nadine S Aguilera (NS)

Department of Pathology, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.

Golam Mohi (G)

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA. gm7sj@virginia.edu.
University of Virginia Cancer Center, Charlottesville, VA, 22908, USA. gm7sj@virginia.edu.

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Classifications MeSH