The emergence of spontaneous coordinated epithelial rotation on cylindrical curved surfaces.


Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
16 Sep 2022
Historique:
entrez: 14 9 2022
pubmed: 15 9 2022
medline: 15 9 2022
Statut: ppublish

Résumé

Three-dimensional collective epithelial rotation around a given axis represents a coordinated cellular movement driving tissue morphogenesis and transformation. Questions regarding these behaviors and their relationship with substrate curvatures are intimately linked to spontaneous active matter processes and to vital morphogenetic and embryonic processes. Here, using interdisciplinary approaches, we study the dynamics of epithelial layers lining different cylindrical surfaces. We observe large-scale, persistent, and circumferential rotation in both concavely and convexly curved cylindrical tissues. While epithelia of inverse curvature show an orthogonal switch in actomyosin network orientation and opposite apicobasal polarities, their rotational movements emerge and vary similarly within a common curvature window. We further reveal that this persisting rotation requires stable cell-cell adhesion and Rac-1-dependent cell polarity. Using an active polar gel model, we unveil the different relationships of collective cell polarity and actin alignment with curvatures, which lead to coordinated rotational behavior despite the inverted curvature and cytoskeleton order.

Identifiants

pubmed: 36103541
doi: 10.1126/sciadv.abn5406
pmc: PMC9473582
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eabn5406

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Auteurs

Alexandros Glentis (A)

Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France.

Carles Blanch-Mercader (C)

Laboratoire Physico Chimie Curie, UMR 168, Institut Curie, PSL Research University, CNRS, Sorbonne Université, 75005 Paris, France.

Lakshmi Balasubramaniam (L)

Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France.

Thuan Beng Saw (TB)

Mechanobiology Institute, National University of Singapore, 5A Engineering Drive 1, Singapore 117411, Singapore.

Joseph d'Alessandro (J)

Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France.

Sebastien Janel (S)

Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, F-59000 Lille, France.

Audrey Douanier (A)

CRBM, Univ Montpellier, CNRS, Montpellier, France.

Benedicte Delaval (B)

CRBM, Univ Montpellier, CNRS, Montpellier, France.

Frank Lafont (F)

Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, F-59000 Lille, France.

Chwee Teck Lim (CT)

Mechanobiology Institute, National University of Singapore, 5A Engineering Drive 1, Singapore 117411, Singapore.
Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Singapore 117583, Singapore.
Institute for Health Innovation and Technology, National University of Singapore, 14 Medical Drive, Singapore 117599, Singapore.

Delphine Delacour (D)

Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France.

Jacques Prost (J)

Laboratoire Physico Chimie Curie, UMR 168, Institut Curie, PSL Research University, CNRS, Sorbonne Université, 75005 Paris, France.
Mechanobiology Institute, National University of Singapore, 5A Engineering Drive 1, Singapore 117411, Singapore.

Wang Xi (W)

Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France.

Benoit Ladoux (B)

Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France.

Classifications MeSH