The collectin SP-A and its trimeric recombinant fragment protect alveolar epithelial cells from the cytotoxic and proinflammatory effects of human cathelicidin
P2X7 channel
alveolar epithelial cells
antimicrobial activity
cathelicidin LL-37
collectin SP-A
cytotoxicity
inflammation
trimeric recombinant fragment
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2022
2022
Historique:
received:
14
07
2022
accepted:
08
08
2022
entrez:
15
9
2022
pubmed:
16
9
2022
medline:
17
9
2022
Statut:
epublish
Résumé
Human cathelicidin (LL-37) is a defense peptide with antimicrobial activity against various pathogens. However, LL-37 can also trigger tissue injury by binding to host cell membranes. The cytotoxic effects of LL-37 may be especially relevant in chronic respiratory diseases characterized by increased LL-37. The aim of this study was to investigate whether the human collectin SP-A and a trimeric recombinant fragment thereof (rfhSP-A) can regulate the activities of LL-37. To this end, we studied the interaction of LL-37 with SP-A and rfhSP-A by intrinsic fluorescence, dynamic light scattering, and circular dichroism, as well as the effects of these proteins on the antimicrobial and cytotoxic activities of LL-37. Both SP-A and rfhSP-A bound LL-37 with high affinity at physiological ionic strength (
Identifiants
pubmed: 36105805
doi: 10.3389/fimmu.2022.994328
pmc: PMC9464622
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antimicrobial Cationic Peptides
0
Collectins
0
Cathelicidins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
994328Informations de copyright
Copyright © 2022 de Tapia, García-Fojeda, Kronqvist, Johansson and Casals.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Front Immunol. 2018 Sep 03;9:1939
pubmed: 30233570
Methods Mol Biol. 2004;261:55-78
pubmed: 15064449
Biochem J. 2007 Sep 15;406(3):479-89
pubmed: 17542781
Sci Rep. 2016 Sep 14;6:32948
pubmed: 27624595
J Immunol. 1999 Jul 1;163(1):387-95
pubmed: 10384140
Biochemistry. 2003 Aug 19;42(32):9532-42
pubmed: 12911295
Respir Res. 2020 Jul 3;21(1):170
pubmed: 32620122
Antimicrob Agents Chemother. 2012 Feb;56(2):845-56
pubmed: 22083479
Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8898-903
pubmed: 12855765
FASEB J. 2010 Oct;24(10):3662-73
pubmed: 20484671
Biochem Biophys Res Commun. 2017 Nov 4;493(1):71-76
pubmed: 28919413
Nat Rev Immunol. 2005 Jan;5(1):58-68
pubmed: 15630429
J Clin Cell Immunol. 2018;9(4):
pubmed: 30123671
Expert Opin Investig Drugs. 2011 Jul;20(7):897-915
pubmed: 21510825
JCI Insight. 2021 Feb 8;6(3):
pubmed: 33554955
Immunobiology. 2017 Feb;222(2):111-118
pubmed: 27793398
Front Immunol. 2022 Apr 04;13:860262
pubmed: 35444643
Am J Respir Crit Care Med. 2004 Nov 1;170(9):1000-5
pubmed: 15271694
J Biomed Sci. 2013 May 01;20:26
pubmed: 23634990
Inflamm Res. 2000 Feb;49(2):73-9
pubmed: 10738945
Br J Pharmacol. 2007 Jul;151(5):571-9
pubmed: 17471177
Mol Immunol. 2019 Aug;112:291-304
pubmed: 31228661
Am J Respir Cell Mol Biol. 2006 Apr;34(4):399-409
pubmed: 16340000
Methods Mol Biol. 2022;2406:113-130
pubmed: 35089553
Respir Med. 2012 Dec;106(12):1680-9
pubmed: 22981321
J Immunol. 2016 Jul 15;197(2):590-8
pubmed: 27271568
Science. 2017 Jun 9;356(6342):1076-1080
pubmed: 28495878
Front Pharmacol. 2018 Feb 01;9:39
pubmed: 29449813
Biophys J. 2011 Jan 5;100(1):108-16
pubmed: 21190662
Am J Physiol Lung Cell Mol Physiol. 2016 Nov 1;311(5):L881-L892
pubmed: 27612964
Am J Respir Cell Mol Biol. 2014 Nov;51(5):604-14
pubmed: 24960334
J Immunol. 1998 Feb 15;160(4):1920-8
pubmed: 9469454
Crit Rev Microbiol. 2021 Mar;47(2):192-205
pubmed: 33455514
Oncotarget. 2017 May 13;8(47):81813-81824
pubmed: 29137224
Proteins. 2012 Feb;80(2):374-81
pubmed: 22095872
J Biol Chem. 1992 Feb 5;267(4):2664-70
pubmed: 1733963
J Biol Chem. 1998 Dec 11;273(50):33115-8
pubmed: 9837875
J Biol Chem. 2003 Oct 31;278(44):43254-60
pubmed: 12913002
Biochim Biophys Acta. 2012 Nov;1818(11):2550-62
pubmed: 22659676
Infect Immun. 1989 Feb;57(2):546-52
pubmed: 2643575
Int J Mol Sci. 2021 Oct 15;22(20):
pubmed: 34681806
J Gen Virol. 2013 Jan;94(Pt 1):40-49
pubmed: 23052388
Colloids Surf B Biointerfaces. 2022 Feb;210:112237
pubmed: 34836708
Biochem J. 1996 Jan 15;313 ( Pt 2):683-9
pubmed: 8573110
Nat Commun. 2017 May 23;8:15504
pubmed: 28534479
Biochim Biophys Acta. 2016 Mar;1858(3):546-66
pubmed: 26556394
Int Immunopharmacol. 2017 Aug;49:6-12
pubmed: 28549244
Biochemistry. 2000 May 30;39(21):6529-37
pubmed: 10828969
Biochem J. 2005 Oct 1;391(Pt 1):115-24
pubmed: 15932345
Pediatr Pathol Mol Med. 2001 Jul-Aug;20(4):249-68
pubmed: 11486733
J Immunol. 2004 Apr 15;172(8):4987-94
pubmed: 15067080
Nature. 2000 Aug 31;406(6799):959-64
pubmed: 10984043
Elife. 2017 Sep 18;6:
pubmed: 28920575
J Allergy Clin Immunol. 2006 Jun;117(6):1328-35
pubmed: 16750994
J Biol Chem. 2008 Nov 7;283(45):30471-81
pubmed: 18765670
Nat Rev Immunol. 2016 May;16(5):321-34
pubmed: 27087664
Front Med (Lausanne). 2020 Sep 18;7:554
pubmed: 33043031
Nat Rev Drug Discov. 2020 May;19(5):311-332
pubmed: 32107480
J Biol Chem. 1998 Feb 6;273(6):3718-24
pubmed: 9452503
Am J Respir Cell Mol Biol. 2011 Mar;44(3):423-9
pubmed: 20508069
J Innate Immun. 2013;5(3):251-60
pubmed: 23235402
Biochem J. 1999 Aug 1;341 ( Pt 3):501-13
pubmed: 10417311
J Dermatol Sci. 2005 Nov;40(2):123-32
pubmed: 15963694
J Biol Chem. 1998 Jun 12;273(24):15183-91
pubmed: 9614132
Sci Rep. 2019 Mar 18;9(1):4780
pubmed: 30886247
J Biol Chem. 2012 Apr 27;287(18):15034-43
pubmed: 22418431
J Immunol. 2015 Aug 15;195(4):1628-36
pubmed: 26163587
J Immunol. 2009 Jul 1;183(1):543-51
pubmed: 19542465
J Biol Chem. 2005 Mar 4;280(9):7659-70
pubmed: 15615713
Biochim Biophys Acta. 2006 Sep;1758(9):1408-25
pubmed: 16716248
Am J Physiol Lung Cell Mol Physiol. 2000 Nov;279(5):L799-805
pubmed: 11053013
Front Immunol. 2022 May 12;13:880961
pubmed: 35634307
J Biol Chem. 1994 Jan 21;269(3):2241-4
pubmed: 8294481
Vaccines (Basel). 2018 Sep 14;6(3):
pubmed: 30223448
Proc Natl Acad Sci U S A. 2021 Jul 6;118(27):
pubmed: 34183393
Physiol Rev. 1997 Oct;77(4):931-62
pubmed: 9354809
Microbiol Mol Biol Rev. 2016 Jun 15;80(3):629-61
pubmed: 27307579
Biochem J. 2014 Jan 15;457(2):263-75
pubmed: 24117320
Front Immunol. 2018 Sep 04;9:1998
pubmed: 30233589
J Immunol. 2013 Dec 1;191(11):5714-21
pubmed: 24174616
Biomater Adv. 2022 Mar;134:112551
pubmed: 35513950
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9541-6
pubmed: 9689116
Biochem J. 1993 Dec 15;296 ( Pt 3):585-93
pubmed: 8280055
Biochemistry. 2002 Nov 26;41(47):14041-53
pubmed: 12437362
Pulm Pharmacol Ther. 2005;18(5):321-7
pubmed: 15939310
Eur Respir J. 2017 Mar 15;49(3):
pubmed: 28298400