Time-dependent in situ structural and cellular aberrations in rabbit cornea in vivo after mustard gas exposure.
Cornea
Endothelium
Epithelium
Haze
Mustard gas keratopathy
Stroma
Sulfur mustard
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
received:
17
05
2022
revised:
23
08
2022
accepted:
07
09
2022
pubmed:
17
9
2022
medline:
28
10
2022
entrez:
16
9
2022
Statut:
ppublish
Résumé
An array of corneal pathologies collectively called mustard gas keratopathy (MGK) resulting from ocular exposure to sulfur mustard (SM) gas are the most prevalent chemical warfare injury. MGK involves chronic ocular discomfort that results in vision impairment. The etiology of MGK remains unclear and poorly understood primarily due to a lack of scientific data regarding structural and cellular changes in different layers of the cornea altered by mustard vapor exposure in vivo. The goals of this study were to (a) characterize time-dependent changes in different layers of corneal epithelium, stroma, and endothelium in live animals in situ by employing state-of-the-art multimodal clinical ophthalmic imaging techniques and (b) determine if SM-induced acute changes in corneal cells could be rescued by a topical eye drop (TED) treatment using in an established rabbit in vivo model. Forty-five New Zealand White Rabbit eyes were divided into four groups (Naïve, TED, SM, and SM + TED). Only one eye was exposed to SM (200 mg-min/m
Identifiants
pubmed: 36113569
pii: S0014-4835(22)00327-X
doi: 10.1016/j.exer.2022.109247
pii:
doi:
Substances chimiques
Mustard Gas
T8KEC9FH9P
Chemical Warfare Agents
0
Ophthalmic Solutions
0
Fluoresceins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
109247Subventions
Organisme : NEI NIH HHS
ID : U01 EY031650
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY030774
Pays : United States
Informations de copyright
Copyright © 2022. Published by Elsevier Ltd.