Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function.
Journal
Cancer gene therapy
ISSN: 1476-5500
Titre abrégé: Cancer Gene Ther
Pays: England
ID NLM: 9432230
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
09
04
2022
accepted:
26
08
2022
revised:
05
08
2022
pubmed:
17
9
2022
medline:
19
1
2023
entrez:
16
9
2022
Statut:
ppublish
Résumé
5-Lipoxygenase (5-LO), the central enzyme in the biosynthesis of leukotrienes, is frequently expressed in human solid malignancies even though the enzyme is not present in the corresponding healthy tissues. There is little knowledge on the consequences of this expression for the tumor cells regarding gene expression and cellular function. We established a knockout (KO) of 5-LO in different cancer cell lines (HCT-116, HT-29, U-2 OS) and studied the consequences on global gene expression using next generation sequencing. Furthermore, cell viability, proliferation, migration and multicellular tumor spheroid (MCTS) formation were studied in these cells. Our results show that 5-LO influences the gene expression and cancer cell function in a cell type-dependent manner. The enzyme affected genes involved in cell adhesion, extracellular matrix formation, G protein signaling and cytoskeleton organization. Furthermore, absence of 5-LO elevated TGFβ
Identifiants
pubmed: 36114329
doi: 10.1038/s41417-022-00531-9
pii: 10.1038/s41417-022-00531-9
pmc: PMC9842508
doi:
Substances chimiques
Arachidonate 5-Lipoxygenase
EC 1.13.11.34
Arachidonate 15-Lipoxygenase
EC 1.13.11.33
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108-123Informations de copyright
© 2022. The Author(s).
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