Patient derived glioma stem cell spheroid reporter assays for live cell high content analysis.

Three dimensional models of cell culture enables researchers to recreate aspects of tumour biology not replicated by traditional two dimensional techniques. Here we describe a protocol to enable automated high throughput phenotypic profiling across panels of patient derived glioma stem cell spheroid models. We demonstrate the use of both live/dead cell end-points and monitor the dynamic changes in the cell cycle using cell lines expressing the FUCCI cell cycle reporter. Together, these assays provide additional insight into the mechanism of action of compound treatments over traditional cell viability assay endpoints.

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Copyright © 2022. Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Neil Carragher reports financial support was provided by Cancer Research UK. Neil Carragher reports financial support was provided by Brain Tumour Charity. Neil Carragher reports a relationship with Cancer Research UK that includes: funding grants and travel reimbursement.