Pseudomonas putida mediates bacterial killing, biofilm invasion and biocontrol with a type IVB secretion system.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
07
10
2021
accepted:
21
07
2022
pubmed:
20
9
2022
medline:
1
10
2022
entrez:
19
9
2022
Statut:
ppublish
Résumé
Many bacteria utilize contact-dependent killing machineries to eliminate rivals in their environmental niches. Here we show that the plant root colonizer Pseudomonas putida strain IsoF is able to kill a wide range of soil and plant-associated Gram-negative bacteria with the aid of a type IVB secretion system (T4BSS) that delivers a toxic effector into bacterial competitors in a contact-dependent manner. This extends the range of targets of T4BSSs-so far thought to transfer effectors only into eukaryotic cells-to prokaryotes. Bioinformatic and genetic analyses showed that this killing machine is entirely encoded by the kib gene cluster located within a rare genomic island, which was recently acquired by horizontal gene transfer. P. putida IsoF utilizes this secretion system not only as a defensive weapon to kill bacterial competitors but also as an offensive weapon to invade existing biofilms, allowing the strain to persist in its natural environment. Furthermore, we show that strain IsoF can protect tomato plants against the phytopathogen Ralstonia solanacearum in a T4BSS-dependent manner, suggesting that IsoF can be exploited for pest control and sustainable agriculture.
Identifiants
pubmed: 36123439
doi: 10.1038/s41564-022-01209-6
pii: 10.1038/s41564-022-01209-6
pmc: PMC9519443
mid: EMS151387
doi:
Substances chimiques
Soil
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1547-1557Subventions
Organisme : Swiss National Science Foundation
ID : 169307
Pays : Switzerland
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022. The Author(s).
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