Translation of a tissue epigenetic signature to circulating free DNA suggests BCAT1 as a potential noninvasive diagnostic biomarker for lung cancer.
BCAT1
Circulating DNA
DNA methylation
Epigenetics
Lung cancer
Noninvasive
Plasma
ddPCR
Journal
Clinical epigenetics
ISSN: 1868-7083
Titre abrégé: Clin Epigenetics
Pays: Germany
ID NLM: 101516977
Informations de publication
Date de publication:
19 09 2022
19 09 2022
Historique:
received:
23
02
2022
accepted:
08
09
2022
entrez:
19
9
2022
pubmed:
20
9
2022
medline:
23
9
2022
Statut:
epublish
Résumé
Lung cancer patients are diagnosed at late stages when curative treatments are no longer possible; thus, molecular biomarkers for noninvasive detection are urgently needed. In this sense, we previously identified and validated an epigenetic 4-gene signature that yielded a high diagnostic performance in tissue and invasive pulmonary fluids. We analyzed DNA methylation levels using the ultrasensitive digital droplet PCR in noninvasive samples in a cohort of 83 patients. We demonstrated that BCAT1 is the candidate that achieves high diagnostic efficacy in circulating DNA derived from plasma (area under the curve: 0.85). Impact of potentially confounding variables was also explored.
Identifiants
pubmed: 36123616
doi: 10.1186/s13148-022-01334-3
pii: 10.1186/s13148-022-01334-3
pmc: PMC9487112
doi:
Substances chimiques
Biomarkers, Tumor
0
Cell-Free Nucleic Acids
0
DNA
9007-49-2
BCAT1 protein, human
EC 2.6.1.
Transaminases
EC 2.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
116Informations de copyright
© 2022. The Author(s).
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