Myofibroblast-dominant proliferation associated with severe fibrosis in bulbar urethral strictures.
anastomotic urethroplasty
bulbar urethral stricture
immunohistochemistry
myofibroblast
Journal
International journal of urology : official journal of the Japanese Urological Association
ISSN: 1442-2042
Titre abrégé: Int J Urol
Pays: Australia
ID NLM: 9440237
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
25
03
2022
accepted:
04
09
2022
pubmed:
21
9
2022
medline:
31
1
2023
entrez:
20
9
2022
Statut:
ppublish
Résumé
Myofibroblast-dominant proliferation (relative to fibroblast proliferation) is the key process in urethral fibrosis, but its association with clinical features is not understood. We conducted a histological analysis of urethral strictures and examined the association between myofibroblast proliferation and stricture characteristics. Formalin-fixed, paraffin-embedded urethral sections sliced axially from 175 male patients with bulbar urethral strictures were retrospectively analyzed. All patients underwent excision and primary anastomosis between September 2008 and January 2021 by a surgeon (AH). Masson's trichrome stain was used to estimate the area of fibrosis. Corresponding unstained slides with the largest area of fibrosis were selected and double-immunostained with anti-smooth muscle actin (SMA) and anti-TE-7 mouse monoclonal antibodies for the assessment of myofibroblasts and fibroblasts, respectively. The ratio of the number of SMA-positive cells to the number of TE-7-positive cells (SMA/TE-7 ratio) was calculated. The area of fibrosis in strictures due to perineal trauma (n = 85, median 108.9 mm Myofibroblast-dominant proliferation may contribute to the pathogenesis of severe urethral fibrosis.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107-112Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022 The Japanese Urological Association.
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