An elderberry-supplemented diet improves spermatogenesis in mice with busulfan-induced azoospermia.

Approximately 40-50% of all infertility cases are due to male infertility, and one of the most important causes of infertility is azoospermia. This study aimed to evaluate the potential effect of elderberry on the spermatogenesis process in the azoospermia mice model. Thirty adult male mice were randomised into three groups: control; busulfan (45mg/kg); and busulfan+elderberry (2%), 6mL orally per animal. Sperm samples were collected from the tail of the epididymis, and testis specimens were also collected and then subjected to sperm parameters analysis, histopathological evaluation, reactive oxygen species (ROS), and glutathione (GSH) measurement to determine the mRNA expression and hormonal assay. It can be concluded that the elderberry diet may be considered a complementary treatment to improve the spermatogenesis process in busulfan-induced azoospermic mice. Considering some limitations, the elderberry diet can be an alternate option for improving testicular damage following chemotherapy.