Online 2D Fluorescence Monitoring in Microtiter Plates Allows Prediction of Cultivation Parameters and Considerable Reduction in Sampling Efforts for Parallel Cultivations of

2D fluorescence spectroscopy high-throughput microtiter plate multivariate data analysis online monitoring

Journal

Bioengineering (Basel, Switzerland)
ISSN: 2306-5354
Titre abrégé: Bioengineering (Basel)
Pays: Switzerland
ID NLM: 101676056

Informations de publication

Date de publication:
04 Sep 2022
Historique:
received: 02 08 2022
revised: 15 08 2022
accepted: 02 09 2022
entrez: 22 9 2022
pubmed: 23 9 2022
medline: 23 9 2022
Statut: epublish

Résumé

Multi-wavelength (2D) fluorescence spectroscopy represents an important step towards exploiting the monitoring potential of microtiter plates (MTPs) during early-stage bioprocess development. In combination with multivariate data analysis (MVDA), important process information can be obtained, while repetitive, cost-intensive sample analytics can be reduced. This study provides a comprehensive experimental dataset of online and offline measurements for batch cultures of

Identifiants

pubmed: 36134983
pii: bioengineering9090438
doi: 10.3390/bioengineering9090438
pmc: PMC9495725
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Innovative Medicines Initiative 2 Joint Undertaking
ID : 777397

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Auteurs

Christoph Berg (C)

AVT-Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074 Aachen, Germany.

Nina Ihling (N)

AVT-Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074 Aachen, Germany.

Maurice Finger (M)

AVT-Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074 Aachen, Germany.

Olivier Paquet-Durand (O)

Department of Process Analytics & Cereal Science, Institute for Food Science and Biotechnology, University of Hohenheim, Garbenstraße 23, 70599 Stuttgart, Germany.

Bernd Hitzmann (B)

Department of Process Analytics & Cereal Science, Institute for Food Science and Biotechnology, University of Hohenheim, Garbenstraße 23, 70599 Stuttgart, Germany.

Jochen Büchs (J)

AVT-Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074 Aachen, Germany.

Classifications MeSH