Colitis caused by Entamoeba histolytica identified by real-time-PCR and fluorescence in situ hybridization from formalin-fixed, paraffin-embedded tissue.
Entamoeba histolytica
PCR
colitis
fluorescence in situ hybridization
formalin
paraffin
Journal
European journal of microbiology & immunology
ISSN: 2062-509X
Titre abrégé: Eur J Microbiol Immunol (Bp)
Pays: Hungary
ID NLM: 101569896
Informations de publication
Date de publication:
22 Sep 2022
22 Sep 2022
Historique:
received:
12
08
2022
accepted:
04
09
2022
entrez:
22
9
2022
pubmed:
23
9
2022
medline:
23
9
2022
Statut:
aheadofprint
Résumé
Intestinal amoebiasis in a 35-year-old German patient with a 3 weeks travel history in Indonesia was initially misidentified as non-steroidal anti-inflammatory-drug associated colitis in colonoscopy and histopathological analysis. Furthermore, initial stool examination by microscopy and Entamoeba faecal antigen ELISA did not reveal any protozoan infection. When cessation of non-steroidal anti-inflammatory drug (NSAID) use and mesalazine treatment did not lead to clinical improvement, the patient presented to a specialist for tropical diseases. An intensive reinvestigation including a workup of formalin-fixed, paraffin-embedded colonic biopsies by molecular analysis with real-time PCR and fluorescence in situ hybridization (FISH) proofed the diagnosis of Entamoeba histolytica colitis. Molecular methods including real-time PCR and FISH for the diagnosis of amoebiasis from histopathological samples are rarely used for the diagnosis of E. histolytica infections. Bloody diarrhoea vanished after the onset of metronidazole treatment. In conclusion, the here-presented case demonstrates how modern molecular diagnostics may help to diagnose E. histolytica-associated colitis, even from difficult specimens like paraffin-embedded, formalin-fixed tissue.
Identifiants
pubmed: 36136732
doi: 10.1556/1886.2022.00016
pmc: PMC9530678
doi:
Types de publication
Case Reports
Langues
eng
Références
Gut. 1969 Apr;10(4):270-7
pubmed: 4305709
Mol Pathol. 2002 Dec;55(6):398-400
pubmed: 12456780
Nucleic Acids Res. 2004 Feb 25;32(4):1363-71
pubmed: 14985472
Am J Trop Med Hyg. 2011 Feb;84(2):332-7
pubmed: 21292910
Environ Microbiol. 2008 Oct;10(10):2894-8
pubmed: 18647333
Pathologica. 2012 Feb;104(1):34-7
pubmed: 22799054
Gut. 1994 Aug;35(8):1018-21
pubmed: 7926897
Am J Proctol. 1973 Aug;24(4):326-32 passim
pubmed: 4354439
Am J Gastroenterol. 1991 Aug;86(8):1063-5
pubmed: 1858742
J Clin Microbiol. 2011 Jan;49(1):257-62
pubmed: 21048004
Dis Colon Rectum. 1977 Mar;20(2):149-53
pubmed: 191233
J Infect Dis. 1997 Mar;175(3):734-6
pubmed: 9041357
J Clin Microbiol. 2005 May;43(5):2168-72
pubmed: 15872237
Eur J Gastroenterol Hepatol. 2004 Aug;16(8):775-8
pubmed: 15256979
Cesk Patol. 2020 Spring;56(2):95-98
pubmed: 32493026
Ann Trop Med Parasitol. 2004 Jan;98(1):43-8
pubmed: 15000730
Gut. 2006 Jun;55(6):832, 841
pubmed: 16698754
mBio. 2018 Sep 18;9(5):
pubmed: 30228239
J Clin Microbiol. 1997 Sep;35(9):2405-7
pubmed: 9276425
J Clin Microbiol. 2000 Feb;38(2):830-8
pubmed: 10655393
Ann Intern Med. 2013 Mar 19;158(6):456-68
pubmed: 23552375
Mod Rheumatol Case Rep. 2022 Jun 24;6(2):270-272
pubmed: 35388431
J Clin Microbiol. 2000 Feb;38(2):818-25
pubmed: 10655391
Scand J Gastroenterol. 2018 Sep;53(9):1085-1088
pubmed: 30256685
Springerplus. 2016 Sep 15;5(1):1586
pubmed: 27652159
Trop Med Int Health. 2004 May;9(5):651-2
pubmed: 15117312
J Glob Infect Dis. 2011 Apr;3(2):166-74
pubmed: 21731305
Jpn J Infect Dis. 2022 May 24;75(3):262-268
pubmed: 34588367
Parasitol Today. 1998 May;14(5):181-5
pubmed: 17040747
Pathol Res Pract. 2001;197(4):271-4; discussion 275-8
pubmed: 11358014
Mol Biol Evol. 1999 Dec;16(12):1740-51
pubmed: 10605115
J Clin Microbiol. 2002 Dec;40(12):4413-7
pubmed: 12454128
Int J Surg Pathol. 2009 Apr;17(2):116-21
pubmed: 18499688
Pathol Res Pract. 2020 Jan;216(1):152608
pubmed: 31564573
Dis Colon Rectum. 1972 Mar-Apr;15(2):147-9
pubmed: 4337386
Rev Esp Enferm Dig. 2017 Jan;109(1):64-65
pubmed: 28100059
Am J Trop Med Hyg. 2010 Oct;83(4):909-13
pubmed: 20889890
Wiad Lek. 1994 Apr;47(7-8):248-51
pubmed: 7941573
J Parasitol. 2010 Aug;96(4):793-7
pubmed: 20496961
Gastrointest Endosc. 2010 Feb;71(2):400-1; discussion 401
pubmed: 19863958
Aust N Z J Surg. 1964 Feb;33(3):194-9
pubmed: 4322103
Eur J Clin Microbiol Infect Dis. 2009 Sep;28(9):1045-53
pubmed: 19415354
PLoS Negl Trop Dis. 2016 Jul 28;10(7):e0004879
pubmed: 27467600
Arch Roum Pathol Exp Microbiol. 1981 Jan-Mar;40(1):85-90
pubmed: 6309117
J Clin Microbiol. 2004 Mar;42(3):1220-3
pubmed: 15004079
J Clin Gastroenterol. 1989 Aug;11(4):407-10
pubmed: 2547865
Digestion. 2010;82(1):18-23
pubmed: 20145404
Kansenshogaku Zasshi. 2016 Jan;90(1):73-6
pubmed: 27032177
Trop Med Int Health. 2003 Dec;8(12):1153-6
pubmed: 14641852
Am J Trop Med Hyg. 2007 Apr;76(4):713-7
pubmed: 17426176
J Gastroenterol Hepatol. 2007 Jan;22(1):64-7
pubmed: 17201883
Clin Microbiol Infect. 2006 Aug;12(8):815-8
pubmed: 16842583
Singapore Med J. 1995 Jun;36(3):303-5
pubmed: 8553098
Sci Rep. 2022 Jun 13;12(1):9730
pubmed: 35697847
Clin Microbiol Infect. 2009 Sep;15(9):869-74
pubmed: 19624500
World J Gastroenterol. 2003 Aug;9(8):1834-5
pubmed: 12918132
PLoS Negl Trop Dis. 2019 Nov 14;13(11):e0007744
pubmed: 31725715
Crit Rev Microbiol. 2017 May;43(3):263-293
pubmed: 28129707
BMC Bioinformatics. 2006 May 04;7:240
pubmed: 16672074
J Clin Gastroenterol. 1992 Jan;14(1):91-2
pubmed: 1556420
Methods. 2001 Dec;25(4):419-29
pubmed: 11846611
Infez Med. 2017 Sep 1;25(3):281-284
pubmed: 28956549
Gastroenterology. 2019 Dec;157(6):1483-1484
pubmed: 31310741
PLoS One. 2019 Feb 21;14(2):e0212791
pubmed: 30789955
Arch Intern Med. 1975 May;135(5):681-5
pubmed: 1052663
Trends Parasitol. 2003 Nov;19(11):523-6
pubmed: 14580964
Diagn Mol Pathol. 2013 Dec;22(4):236-44
pubmed: 24193008
Turk J Gastroenterol. 2006 Jun;17(2):130-2
pubmed: 16830298
Ann Gastroenterol Hepatol (Paris). 1989 Jun-Sep;25(4):137-40
pubmed: 2764495
Schweiz Med Wochenschr. 1999 Nov 6;129(44):1656-9
pubmed: 10588024
Frontline Gastroenterol. 2013 Jan;4(1):32-40
pubmed: 23914292
Rev Bras Reumatol. 2014 Nov-Dec;54(6):483-5
pubmed: 25458030
Travel Med Infect Dis. 2021 May-Jun;41:102042
pubmed: 33812018
JMM Case Rep. 2016 Jun 10;3(3):e005032
pubmed: 28348756
Z Gesamte Inn Med. 1990 Jan 1;45(1):25-7
pubmed: 2330735
Transpl Infect Dis. 2015 Dec;17(6):886-9
pubmed: 26426525
AJR Am J Roentgenol. 1977 Jun;128(6):935-41
pubmed: 414558
BMJ Case Rep. 2015 Nov 25;2015:
pubmed: 26607188
Acta Trop. 2020 Jul;207:105516
pubmed: 32371221
Ugeskr Laeger. 1993 Sep 13;155(37):2901-2
pubmed: 8259618
Microbiol Rev. 1995 Mar;59(1):143-69
pubmed: 7535888
South Med J. 2015 Nov;108(11):676-81
pubmed: 26539949