Shared Clavulanate and Tazobactam Antigenic Determinants Activate T-Cells from Hypersensitive Patients.


Journal

Chemical research in toxicology
ISSN: 1520-5010
Titre abrégé: Chem Res Toxicol
Pays: United States
ID NLM: 8807448

Informations de publication

Date de publication:
21 11 2022
Historique:
pubmed: 23 9 2022
medline: 23 11 2022
entrez: 22 9 2022
Statut: ppublish

Résumé

β-Lactamase inhibitors such as clavulanic acid and tazobactam were developed to overcome β-lactam antibiotic resistance. Hypersensitivity reactions to these drugs have not been studied in detail, and the antigenic determinants that activate T-cells have not been defined. The objectives of this study were to (i) characterize clavulanate- and tazobactam-responsive T-cells from hypersensitive patients, (ii) explore clavulanate and tazobactam T-cell crossreactivity, and (iii) define the antigenic determinants that contribute to T-cell reactivity. Antigen specificity, pathways of T-cell activation, and crossreactivity with clavulanate- and tazobactam-specific T-cell clones were assessed by proliferation and cytokine release assays. Antigenic determinants were analyzed by mass spectrometry-based proteomics methods. Clavulanate- and tazobactam-responsive CD4

Identifiants

pubmed: 36137197
doi: 10.1021/acs.chemrestox.2c00231
pmc: PMC9682523
doi:

Substances chimiques

Clavulanic Acid 23521W1S24
Tazobactam SE10G96M8W
Epitopes 0
beta-Lactamase Inhibitors 0
Anti-Bacterial Agents 0
Aldehydes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2122-2132

Subventions

Organisme : Medical Research Council
ID : MR/L006758/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R009635/1
Pays : United Kingdom

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Auteurs

Adriana Ariza (A)

Allergy Research Group, Instituto de Investigación Biomédica de Málaga-IBIMA, 29009 Málaga, Spain.

Kanoot Jaruthamsophon (K)

Department of Pharmacology and Therapeutics, Institute of Systems, Molecular, and Integrative Biology, University of Liverpool, Liverpool L69 3GE, U.K.
Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.

Xiaoli Meng (X)

Department of Pharmacology and Therapeutics, Institute of Systems, Molecular, and Integrative Biology, University of Liverpool, Liverpool L69 3GE, U.K.

Marina Labella (M)

Allergy Research Group, Instituto de Investigación Biomédica de Málaga-IBIMA, 29009 Málaga, Spain.
Allergy Unit, Hospital Regional Universitario de Málaga, 29009 Málaga, Spain.

Kareena Adair (K)

Department of Pharmacology and Therapeutics, Institute of Systems, Molecular, and Integrative Biology, University of Liverpool, Liverpool L69 3GE, U.K.

Arun Tailor (A)

Department of Pharmacology and Therapeutics, Institute of Systems, Molecular, and Integrative Biology, University of Liverpool, Liverpool L69 3GE, U.K.

Chonlaphat Sukasem (C)

Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.

Paul Whitaker (P)

Bradford Teaching Hospitals NHS Trust, Bradford BD9 6DA, U.K.

Daniel Peckham (D)

Regional Adult Cystic Fibrosis Unit, St James's University Hospital, Leeds LS9 7TF, U.K.

Munir Pirmohamed (M)

Department of Pharmacology and Therapeutics, Institute of Systems, Molecular, and Integrative Biology, University of Liverpool, Liverpool L69 3GE, U.K.

María José Torres (MJ)

Allergy Research Group, Instituto de Investigación Biomédica de Málaga-IBIMA, 29009 Málaga, Spain.
Allergy Unit, Hospital Regional Universitario de Málaga, 29009 Málaga, Spain.
Andalusian Center for Nanomedicine and Biotechnology-BIONAND, 29590 Málaga, Spain.
Departamento de Medicina, Universidad de Málaga, 29071 Málaga, Spain.

Dean John Naisbitt (DJ)

Department of Pharmacology and Therapeutics, Institute of Systems, Molecular, and Integrative Biology, University of Liverpool, Liverpool L69 3GE, U.K.

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Classifications MeSH